Development of TaqMan Fluorescence Quantitative PCR Assay for Detection of Chicken Infectious Anemia Virus |
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| Authors: | GUO Cui-li ZHANG Chun-hong GUO Peng-ju ZHANG Jian-feng SHEN Hai-yan PAN Jia-qiang |
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| Institution: | 1. South China Agricultural University, Guangzhou 510640, China;2. Institute of Animal Health, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China |
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| Abstract: | A fluorescence quantitative PCR assay was developed using primers and probes designed according to a high conservative sequence of chicken infectious anemia virus (CAV). Detection of viral DNA copy number was determined by extrapolation from a CAV plasmid-based standard curve. The results showed that the estimated average viral copy number could detect 1.8×101 copies/μL of plasmid DNA with high reproducibility (CAV of the intra or inter) assay was below 2%. Furthermore, 1 day-old SPF chicken were inoculated with CAV and the virus loads were detected in multiple organs. The results showed that the virus could be detected in the brain, heart, liver, spleen, lung, kidney, thymus, bursa and serum and the virus loads in these organs showed no significant difference. No cross-reaction was detected with other avian virus. |
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| Keywords: | chicken infectious anemia virus TaqMan Real-time PCR probe |
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