Effect of grape seed proanthocyanidin on cisplatin-induced nephrotoxicity in mice |
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| Authors: | GAO Zhao-li LIU Guang-yi LI Xing HU Zhao YANG Xiang-dong JIANG Bei LI Xian-hua |
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| Institution: | Department of Nephrology, Qilu Hospital of Shandong University, Jinan 250012, China. E-mail: |
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| Abstract: | AIM:To study the protective effect of grape seed proanthocyanidin (GSP) on nephrotoxicity induced by cisplatin (CP) in mice. METHODS:Male C57 mice were randomly divided into 4 groups: control group (N group, n=10), CP group (intraperitoneal injection of CP at dose of 20 mg/kg, n=20), GSP group (intragastric administration of GSP at dose of 500 mg/kg, n=15) and CP+GSP group (intragastric administration of GSP 30 min prior to intraperitoneal injection of CP and intragastric administration of the same dose of GSP 72 h later, n=20). On the 5th day after CP treatment, blood and kidney samples were collected. The renal pathological changes were examined by HE staining. The protein expression of glucose-regulated protein 78 (GRP78) and phosphorylated extracellular signal-regulated kinase (p-ERK) was examined by Western blotting and immunohistochemical staining. RESULTS:Renal index, blood urea nitrogen and serum creatinine were significantly higher in CP group than those in N group. The renal tissues were heavily damaged in CP group. The protein expression of GRP78 and p-ERK increased in CP group compared with N group. GSP treatment alleviated the increase in the renal index, blood urea nitrogen and serum creatinine. The damages of the renal tissues were attenuated. The protein expression of GRP78 and p-ERK was obviously reduced in CP+GSP group compared with CP group. CONCLUSION:GSP prevents kidney from CP-induced damage by suppression of endoplasmic reticulum stress. |
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| Keywords: | Cisplatin Grape seed proanthocyanidin Nephrotoxicity Endoplasmic reticulum stress |
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