| 猪繁殖与呼吸综合征病毒分离株GP5蛋白ORF5基因的克隆与序列分析 |
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| 引用本文： | 吴晓薇,刘晓慧,杨谦,熊蕊,郭凤柳,郭霄峰,阳佑天,陈茹.猪繁殖与呼吸综合征病毒分离株GP5蛋白ORF5基因的克隆与序列分析[J].中国畜牧兽医,2014,41(11):40-43. |
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| 作者姓名： | 吴晓薇 刘晓慧 杨谦 熊蕊 郭凤柳 郭霄峰 阳佑天 陈茹 |
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| 作者单位： | 1. 广东出入境检验检疫局, 广东广州 510642;2. 保定出入境检验检疫局, 河北保定 071051;3. 华南农业大学, 广东广州 510640 |
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| 基金项目： | 广东省科技计划项目(2011B020306004)。 |
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| 摘 要： | 为揭示广东地区猪繁殖与呼吸综合征(porcine reproductive and respiratory syndrome,PRRS)可能的流行规律,本研究采用RT-PCR的方法扩增5株来自广东地区猪场临床样品的猪繁殖与呼吸综合征病毒(porcine reproductive and respiratory syndrome virus, PRRSV)分离株的GP5蛋白ORF5基因,将其克隆、测序后与国内外部分毒株相应基因进行核苷酸序列比较。结果表明,PRRSV-2011-GD2、PRRSV-2011-GD3、PRRSV-2013-GD1、PRRSV-2013-GD2和PRRSV-2013-GD3株与美洲型参考株(VR-2332) 的核苷酸序列同源性分别为88.8%、99.5%、88.7%、88.7%和83.5%。系统进化树分析结果表明,PRRSV-2011-GD2、PRRSV-2011-GD3、PRRSV-2013-GD1、PRRSV-2013-GD2和PRRSV-2013-GD3株均属于美洲型。以上结果为更好地了解PRRSV的分子流行病学特征和抗原变异规律提供依据, 同时为研制基因工程疫苗奠定了基础。
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| 关 键 词： | 猪繁殖与呼吸综合征 GP5蛋白 序列分析 |
| 收稿时间： | 2014-04-04 |
Cloning and Sequence Analysis of GP5 Genes of Porcine Reproductive and Respiratory Syndrome Virus Prevalence Strains |
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| WU Xiao-wei,LIU Xiao-hui,YANG Qian,XIONG Rui,GUO Feng-liu,GUO Xiao-feng,YANG You-tian,CHEN Ru.Cloning and Sequence Analysis of GP5 Genes of Porcine Reproductive and Respiratory Syndrome Virus Prevalence Strains[J].China Animal Husbandry & Veterinary Medicine,2014,41(11):40-43. |
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| Authors: | WU Xiao-wei LIU Xiao-hui YANG Qian XIONG Rui GUO Feng-liu GUO Xiao-feng YANG You-tian CHEN Ru |
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| Institution: | 1. Guangdong Entry-exit Inspection and Quarantine Bureau, Guangzhou 510642, China;2. Baoding Entry-exit Inspection and Quarantine Bureau, Baoding 071051, China;3. South China Agricultural University, Guangzhou 510640, China |
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| Abstract: | In order to reveal the Guangdong region of porcine reproductive and respiratory syndrome (PRRS) epidemic law, 5 strains of swine clinical samples amplified from Guangdong region of PRRSV GP5 genes of the isolates with the method of RT-PCR, cloned, sequenced and domestic and foreign strains corresponding gene nucleotide sequence comparison in this study.The results showed that PRRSV-2011-GD2, PRRSV-2011-GD3, PRRSV-2013-GD1, PRRSV-2013-GD2 and PRRSV-2013-GD3 strains with the American type reference strains (ATCC VR-2332 U87392) nucleotide sequence homology were 88.8%, 99.5%, 88.7%, 88.7% and 83.5%,respectively. The phylogenetic tree analysis indicated that PRRSV-2011-GD2, PRRSV-2011-GD3, PRRSV-2013-GD1, PRRSV-2013-GD2 and PRRSV-2013-GD3 strains belonged to the American.The results provided the basis for a better understanding of the molecular epidemiology of PRRSV features and antigenic variation law, also laid the foundation for the study of gene engineering vaccine. |
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| Keywords: | porcine reproductive and respiratory syndrome GP5 protein sequence analysis |
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