| 丙戊酸处理对猪手工克隆重构胚体外发育潜能的影响 |
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| 引用本文： | 谢炳坤,吕玲燕,陆杏蓉,崔奎青,孙俊铭,覃兆鲜,石德顺,刘庆友.丙戊酸处理对猪手工克隆重构胚体外发育潜能的影响[J].中国畜牧兽医,2014,41(5):180-186. |
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| 作者姓名： | 谢炳坤 吕玲燕 陆杏蓉 崔奎青 孙俊铭 覃兆鲜 石德顺 刘庆友 |
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| 摘 要： | 为探讨组蛋白去乙酰化酶抑制剂丙戊酸（valproic acid, VPA)对猪手工克隆(HMC)胚胎发育潜能的影响，本研究比较了不同浓度（0、25、50、75和100 nmol/L）VPA处理猪HMC重构胚对后期胚胎发育率、内细胞团数和组蛋白乙酰化程度的影响。结果表明，50、75 nmol/L VPA处理组HMC重构胚的卵裂率和囊胚发育率均显著高于0、25和100 nmol/L VPA处理组（P<0.05）；与0、25、75和100 nmol/L相比，50 nmol/L VPA处理组能显著提高囊胚内细胞团细胞数（P<0.05），囊胚阶段VPA处理组的组蛋白H3K14乙酰化（AcH3K14）水平高于空白组和孤雌激活囊胚。因此，应用VPA处理可提高猪HMC重构胚胎分裂率、囊胚率及增加内细胞团细胞数，提高了猪HMC胚胎的体外发育潜能。
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| 收稿时间： | 2013-10-30 |
Effect of Valproic Acid on in vitro Developmental Competence of Handmade Cloned Pig Reconstructed Embryos |
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| XIE Bing-kun,LV Ling-yan,LU Xing-rong,CUI Kui-qing,SUN Jun-ming,QIN Zhao-xian,SHI De-shun,LIU Qing-you.Effect of Valproic Acid on in vitro Developmental Competence of Handmade Cloned Pig Reconstructed Embryos[J].China Animal Husbandry & Veterinary Medicine,2014,41(5):180-186. |
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| Authors: | XIE Bing-kun LV Ling-yan LU Xing-rong CUI Kui-qing SUN Jun-ming QIN Zhao-xian SHI De-shun LIU Qing-you |
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| Abstract: | To explore the effects of histone deaceylase inhibitors valproic acid (VPA) affecting the development potency of handmade cloned (HMC) embryos, porcine HMC reconstructed embryos were cultured for 24 h with different concentration (0，25，50，75 and 100 nmol/L) of VPA, and the blastocyst development rate, inner cell mass (ICM) cells number and histone acetylation level were compared. The results showed that reconstructed embryos were cultured in PZM-3 containing 50， 75 nmol/L VPA for 24 h, the frequency of the rate of cleavage and blastocyst formation were significantly higher in embryos treated with VPA than 0, 25, 100 nmol/L VPA treatments （P＜0.05）. In addition, 50 nmol/L VPA treatment significantly increased the number of inner cell mass (ICM) cells compared with the other groups (P＜0.05). The AcH3K14 level in embryos treated with VPA was higher than the control group and parthenogenetic activation group. In conclusion, epigenetic modification VPA could enhances the rate of cleavage, blastocyst formation and the ICM cells number of porcine HMC reconstructed embryos, which mean improve the developmental potency of HMC embryos. |
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