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| 拟穴青蟹血细胞原代培养及条件优化 | |
| 引用本文： | 乔琨,张亚群,王书平,安哲,郝华,陈芳奕,彭会.拟穴青蟹血细胞原代培养及条件优化[J].中国畜牧兽医,2014,41(9):145-149. |
| 作者姓名： | 乔琨 张亚群 王书平 安哲 郝华 陈芳奕 彭会 |
| 作者单位： | 1. 厦门大学海洋与地球学院, 福建厦门 361102; 2. 福建省海洋生物制备技术工程实验室, 福建厦门 361102 |
| 基金项目： | 福建省农业科技重点项目（2012N0029）；中国博士后科学基金面上资助（2014M550354）。 |
| 摘 要： | 本研究通过对拟穴青蟹血细胞原代培养条件的摸索与优化，成功在体外培养了青蟹血细胞。结果表明，pH 4.6的海洋甲壳类血细胞抗凝剂培养青蟹血细胞的形态优于海洋蟹类抗凝剂（ACD）；高血清浓度（>15%）培养血细胞形态较差，易出现空泡；添加5% FBS与1.2% NaCl的L15培养基是培养拟穴青蟹血细胞最适培养基，并可在培养的青蟹原代血细胞中检测到稳定的管家基因表达，表明该细胞可用于拟穴青蟹体外功能试验研究。 |
| 关 键 词： | 拟穴青蟹 血细胞 原代培养 |
The Optimization of Primary Hemocyte Culture of Scylla paramamosain |
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| QIAO Kun,ZHANG Ya-qun,WANG Shu-ping,AN Zhe,HAO Hua,CHEN Fang-yi,PENG Hui.The Optimization of Primary Hemocyte Culture of Scylla paramamosain[J].China Animal Husbandry & Veterinary Medicine,2014,41(9):145-149. | |
| Authors: | QIAO Kun ZHANG Ya-qun WANG Shu-ping AN Zhe HAO Hua CHEN Fang-yi PENG Hui |
| Institution: | 1. College of Ocean and Earth Science, Xiamen University, Xiamen 361102, China; 2. Fujian Engineering Laboratory of Marine Bioproducts and Technology, Xiamen 361102, China |
| Abstract: | The optimization of primary hemocyte culture of Scylla paramamosain was successfully studied in vitro.The results showed that the hemocyte cells were morphologically kept activity better at pH 4.6 marine crustaceans anticoagulant.High concentration of fetal bovine serum (>15%) in the culture lead to development of vacuoles.The hemocyte cells survived best in L15 medium supplemented with 5% fetal bovine serum and 1.2% sodium chloride.The house keeping gene β-actin of Scylla paramamosain of cultured hemocyte maintained stably expressed through the experiment.This cell culture system could be used for in vitro experiments. |
| Keywords: | Scylla paramamosain hemocyte primary culture |
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