Screening the GHSR Gene for SNPs and Analyzing the Mutations in Guizhou Semi-fine Wool Sheep |
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| Authors: | ZHANG Qiong-di SONG De-rong ZHOU Da-rong PENG Hua LIU Ruo-yu WU Rui-ru JIANG Xing-mei WU Ping |
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| Institution: | （1.Animal Husbandry and Veterinary Science Institute of Bijie City, Bijie 551700，China；2.College of Animal Science, Guizhou University, Huaxi 550025，China） |
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| Abstract: | By studying the GHSR gene polymorphism, A basis of selection and breeding was provide on molecular biology of Guizhou Semi-fine Wool sheep. Guizhou Semi-fine Wool sheep were used to construct DNA pools in this study, the complete sequences of exon 2 and part of intron 1 and intron 2 region in GHSR gene were obtained by designing one pair of specific primers. The amplified PCR products would be directly purified and conducted bidirectional sequences subsequently. DNAStar and BLAST software were used to identify polymorphisms of GHSR gene. Moreover, the impact of SNPs on secondary structure of RNA, secondary structure of GHSR protein would be analyzed by the bioinformatics software. The results showed that two SNPs (T70G and G229A) were found in GHSR gene which were two synonymous mutations. The SNPs led to the changes of RNA secondary structure. GHSR gene polymorphism might affect the growth of Guizhou Semi-fine Wool sheep. |
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