Levels of PEDF and TNF-α in PBMCs of type 2 diabetic retinopathy patients |
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| Authors: | L Qiu-ju TANG Xi-xiang QIU Ya-wei ZENG Ke-jing MU Pan-wei CHEN Yan-ming ZENG Long-yi |
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| Institution: | Department of Endocrinology & Metabolism, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou 510630, China. |
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| Abstract: | AIM:To investigate the roles of pigment epithelium-derived factor (PEDF), phosphorylated NF-κB p65 and TNF-α in peripheral blood mononuclear cells (PBMCs) in diabetic retinopathy (DR) patients. METHODS:Fifty-two healthy persons were enrolled in the study as normal control group (NC group). Type 2 diabetic patients served as DM group (n=108), which were sub-divided into non-diabetic retinopathy group (NDR group, n=52) and diabetic retinopathy group (DR group, n=56) by angiography. The PBMCs were isolated by the technique of density-gradient centrifugation. The protein levels of PEDF, phosphorylated NF-κB p65 and TNF-α in the PBMCs were determined by Western blotting. The levels of plasma PEDF and TNF-α were detected by ELISA. The content of serum uric acid (SUA) and white blood cell count were measured. RESULTS:The levels of PEDF, TNF-α and phosphorylated NF-κB p65 in the PBMCs were statistically higher in NDR group and DR group than those in control group. The level of TNF-α increased significantly in DR group as compared with NDR group. The levels of PEDF and phosphorylated NF-κB p65 were slightly but not significantly higher in DR group than those in NDR group. The plasma levels of PEDF and TNF-α were evidently elevated in NDR group and DR group compared with NC group, and those were obviously higher in DR group than those in NDR group. In the diabetic patients, the plasma level of PEDF was positively correlated with the levels of TNF-α (r=3.39, P<0.05) and SUA (r=0.25, P<0.05). CONCLUSION:The expression of PEDF in PBMCs is markedly increased, accompanied with the elevation of phosphorylated NF-κB p65 and TNF-α in type 2 diabetic patients especially with DR, suggesting that PEDF is possibly involved in the development of DR by inflammatory mechanism. |
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| Keywords: | Pigment epithelium-derived factor Peripheral blood mononuclear cells Diabetic retinopathy |
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