| 山东省肉鸭沙门氏菌的分离鉴定及敏感抗菌药物的筛选 |
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| 引用本文： | 齐静,王守荣,李兰波,骆延波,李璐璐,胡明,王进文,李虹达,贾纪美,赵敏,杨胜福,李玉保,单虎,刘玉庆.山东省肉鸭沙门氏菌的分离鉴定及敏感抗菌药物的筛选[J].中国畜牧兽医,2014,41(7):216-220. |
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| 作者姓名： | 齐静 王守荣 李兰波 骆延波 李璐璐 胡明 王进文 李虹达 贾纪美 赵敏 杨胜福 李玉保 单虎 刘玉庆 |
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| 摘 要： | 从山东省各个地区的病死肉鸭采集317份组织样品进行细菌分离，并进行染色镜检和生化鉴定，共分离鉴定出105株沙门氏菌，分离率为33.1%。将临床推荐用药浓度的0.1倍作为抗药性检测浓度L，设置不同药物浓度梯度（1/4 L、1/2 L、L、2 L和4 L），进行17种抗生素的药敏试验，根据L药物浓度梯度对分离菌株的抑菌率大于80%的原则进行敏感抗菌药物的筛选。结果表明，沙门氏菌是危害山东省肉鸭养殖的主要病原菌之一，且多重耐药现象较严重，其中氨苄西林、诺氟沙星、卡那霉素、氟苯尼考和培氟沙星等敏感药物可作为临床推荐药物对沙门氏菌进行防控。
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Isolation and Identification of Duck Salmonella and Screening of Sensitive Antimicrobials in Shandong Province |
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| QI Jing,WANG Shou-rong,LI Lan-bo LUO Yan-bo,LI Lu-lu,HU Ming,WANG Jin-wen,LI Hong-da,JIA Ji-mei,ZHAO Min,YANG Sheng-fu,LI Yu-bao,SHAN Hu,LIU Yu-qing.Isolation and Identification of Duck Salmonella and Screening of Sensitive Antimicrobials in Shandong Province[J].China Animal Husbandry & Veterinary Medicine,2014,41(7):216-220. |
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| Authors: | QI Jing WANG Shou-rong LI Lan-bo LUO Yan-bo LI Lu-lu HU Ming WANG Jin-wen LI Hong-da JIA Ji-mei ZHAO Min YANG Sheng-fu LI Yu-bao SHAN Hu LIU Yu-qing |
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| Abstract: | 317 tissue samples of sick and dead ducks from various regions of Shandong province were collected for bacterial isolation, staining microscopy and biochemical identification. Totally, 105 strains of Salmonella were identified and the separation rate was 33.1%. Antimicrobial susceptibility testing of all isolates to 17 kinds of antibiotics was carried out by setting up different concentration gradient （1/4 L, 1/2 L, L, 2 L and 4 L） of the clinical recommended dose. The sensitive antimicrobials to the isolates were screened according to the inhibition ratio greater than 80% at the concentration of L gradient. The result showed that Salmonella was one of the main pathogenic bacteria in duck breeding of Shandong province, and with severe multiple drug resistance. Several sensitive antimicrobials including ampicillin, norfloxacin, kanamycin, florfenicol and pefloxacin could be used as clinical recommended drugs for the prevention and control of this disease. |
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