| 新疆野扁桃自交不亲和基因Cullin1的克隆及生物信息学分析 |
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| 引用本文： | 王波,余镇藩,曾 斌,夏江宏,马鑫鑫.新疆野扁桃自交不亲和基因Cullin1的克隆及生物信息学分析[J].中国农学通报,2017,33(34):63-68. |
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| 作者姓名： | 王波 余镇藩 曾 斌 夏江宏 马鑫鑫 |
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| 作者单位： | 新疆农业大学 林学与园艺学院,新疆农业大学 林学与园艺学院,新疆农业大学 林学与园艺学院,新疆农业大学 林学与园艺学院,新疆农业大学 林学与园艺学院 |
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| 基金项目： | 国家自然科学基金“基于S-核酸酶的新疆野生扁桃自交不亲和性泛素化特性的分子机制研究”(31660557)；新疆维吾尔自治区园艺学重点 学科项目“新疆特色果树种质资源开发利用与优良品种选育推广”(2016-10758-3)。 |
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| 摘 要： | 旨在为今后研究新疆野扁桃的分子调控奠定良好基础。通过利用Rt-PCR技术及生物测序等方法,从新疆野扁桃的幼嫩叶片中,克隆得到了一个全新的新疆野扁桃Cullin1基因全长c DNA,命名为Pet Cullin1。并进一步的利用在线软件分析发现:Petcullin1基因全长为2677 bp,其开放阅读框2217 bp,该基因的氨基酸共有738个编码,预测得出Petcullin1蛋白的分子式为C3887H6064N1028O1133S34,原子总数为12096,相对分子质量为85814.61,理论推导出其半衰期为30 h,带正负电荷的残留的总数分别是108和113,不稳定指数和平均疏水指数分别为40.19、-0.452,该蛋白属于水溶性不稳定蛋白。
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| 关 键 词： | 新疆野扁桃 自交不亲和 CUL1基因 克隆 生物信息学 |
| 收稿时间： | 2017/9/13 0:00:00 |
| 修稿时间： | 2017/10/15 0:00:00 |
Self-incompatibility Gene Cullin1 Cloning and Bioinformatics Analysis of Wild Almond in Xinjiang |
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| Abstract: | The aim is to lay a foundation for the future research of molecular regulation of Xinjiang wild almond. A full-length cDNA ofCullin1 gene was cloned from the young leaves of Xinjiang wild almond by using Rt-PCR technique and biological sequencing, and named asPetCullin1 . Further analysis with the online software showed thatPetcullin1 gene was 2677 bp in full-length and its open reading frame was 2217 bp. The amino acid of the gene was predicted to be 738, and the molecular formula of the Petcullin1 protein was predicted to be C3887H6064N1028O1133S34, the total number of atoms was 12096, the relative molecular mass was 85814.61, the theoretical half-time was 30 h. The total number of residues with positive and negative charge was 108 and 113, respectively. The instability index and average hydrophobic index was 40.19 and -0.452 respectively, and the protein belonged to water-soluble labile protein. |
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| Keywords: | wild almond (Amygdalus ledebouriana ) self-incompatibility Cullin1 gene clone bioinformatics |
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