| 猪类乙型肝炎病毒的血清学检测及其S和C基因的克隆与分析 |
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| 引用本文： | 姜中毅,兰喜,李志勇,杨斌,张韵,李学瑞,李宝玉,殷相平,柳纪省.猪类乙型肝炎病毒的血清学检测及其S和C基因的克隆与分析[J].甘肃农业大学学报,2009,44(2). |
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| 作者姓名： | 姜中毅 兰喜 李志勇 杨斌 张韵 李学瑞 李宝玉 殷相平 柳纪省 |
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| 作者单位： | 1. 甘肃农业大学动物医学院,甘肃,兰州,730070;中国农业科学院兰州兽医研究所,家畜疫病病原生物学国家重点实验室,农业部草食动物疫病重点开放实验室,甘肃,兰州,730046
2. 中国农业科学院兰州兽医研究所,家畜疫病病原生物学国家重点实验室,农业部草食动物疫病重点开放实验室,甘肃,兰州,730046 |
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| 摘 要： | 对甘肃省兰州市周边地区采集的300份猪血清的表面抗原(HBsAg)、E抗原(HBeAg)、E抗体(抗-HBe)、核心抗体(抗-HBc)、表面抗体(抗-HBs)5项指标进行检测,并从HBsAg和抗-HBs抗体阳性血清中提取DNA,进行乙型肝炎病毒(HBV)S基因和C基因的巢式PCR扩增、克隆、测序和序列分析.结果表明:血清学检测的上述5项指标阳性率分别为10.7%3、.0%、6.1%、3.3%和88.5%,大三阳(即HBsAg、HBeAg、抗-HBs抗体均为阳性)的猪血清样本6份,阳性率为2%;序列分析表明,猪类HBV的S基因和C基因与GenBank中的人HBV同源性达到99%,说明猪存在类HBV感染,且猪类HBV与人HBV存在相关关系.
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| 关 键 词： | 猪 乙型肝炎病毒 血清学检测 S和C基因 克隆 |
Serological detection and cloning and analysis of S and C genes of porcine hypothesis B virus in swine |
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| JIANG Zhong-yi,LAN Xi,LI Zhi-yong,YANG Bin,ZHANG Yun,LI Xue-rui,LI Bao-yu,YIN Xiang-ping,LIU Ji-xing.Serological detection and cloning and analysis of S and C genes of porcine hypothesis B virus in swine[J].Journal of Gansu Agricultural University,2009,44(2). |
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| Authors: | JIANG Zhong-yi LAN Xi LI Zhi-yong YANG Bin ZHANG Yun LI Xue-rui LI Bao-yu YIN Xiang-ping LIU Ji-xing |
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| Institution: | JIANG Zhong-yi1,2,LAN Xi2,LI Zhi-yong2,YANG Bin2,ZHANG Yun2,LI Xue-rui2,LI Bao-yu2,YIN Xiang-ping2,LIU Ji-xing1,2(1.College of Veterinary Medicine,Gansu Agricultural University,Lanzhou 730070,China,2.Key Laboratory of Herbivorous Animal Disease of Minintry of Agriculture,State Key Laboratory of Veterinary Etiological Biology,Lanzhou Veterinary Research Institute,Chinese Academy of Agriculture Sciences,Lanzhou 730046,China) |
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| Abstract: | In order to investigate the nucleotide mutations of HBV S and C genes in both HBsAg and anti-HBs positive serums of swine,300 serum samples of pigs were used to detect HBsAg,HBeAg,anti-HBs,anti-HBe and anti-HBc levels.DNA was extracted from both HBsAg and anti-HBs positive serums,HBV S gene and C gene were amplified and sequenced.Serological detection revealled that positive rates of HBsAg was 10.7 %(32/300),positive rates of HBeAg was 3.0 %(9/300),positive rates of anti-HBe was 6.1 %(17/280),positive rates... |
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| Keywords: | porcine HBV serological detection S and C gens clone |
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