| 转基因杨树对杨小舟蛾幼虫解毒酶活性的影响 |
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| 引用本文： | 郭同斌,嵇保中,诸葛强,黄敏仁.转基因杨树对杨小舟蛾幼虫解毒酶活性的影响[J].林业科学,2007,43(5):59-63. |
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| 作者姓名： | 郭同斌 嵇保中 诸葛强 黄敏仁 |
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| 作者单位： | 1. 徐州市森林病虫害防治检疫站,徐州,221004
2. 南京林业大学森林资源与环境学院,南京,210037 |
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| 基金项目： | 国家研究发展基金，江苏省高新技术项目 |
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| 摘 要： | 用转Bt单基因和转Bt CpTI双价基因杨树叶片饲喂杨小舟蛾,幼虫中肠酯酶的活力在饲喂初期显著升高,但在饲喂一定时间后受到抑制:转Bt单基因杨树在饲喂24 h开始受到抑制,48 h受到明显抑制,较对照下降了15.8%;而转双价基因杨树在饲喂12 h受到明显抑制,较对照下降了38.1%.转双价基因杨树对中肠酯酶的抑制作用大于转Bt单基因杨树.转Bt单基因杨树对幼虫中肠羧酸酯酶的抑制能力不强,而转双价基因杨树对中肠羧酸酯酶的抑制作用大于转Bt单基因杨树,饲喂12 h后活力开始受到抑制,饲喂24、36、48 h的活力分别较对照下降了33.4%、22.5%、29.6%,与对照均有显著差异.转基因杨树主要通过抑制幼虫中肠酯酶和羧酸酯酶这2种解毒酶活性而干扰昆虫正常的生理代谢,从而起到毒杀害虫的作用.
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| 关 键 词： | 转基因杨树 杨小舟蛾 解毒酶 酯酶 羧酸酯酶 |
| 文章编号： | 1001-7488（2007）05-0059-05 |
| 修稿时间： | 2005-08-19 |
Effect of Transgenic Poplars on the Activities of Detoxification Enzymes in Micromelalopha troglodyta Larvae |
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| Guo Tongbin,Ji Baozhong,Zhuge Qiang,Huang Minren.Effect of Transgenic Poplars on the Activities of Detoxification Enzymes in Micromelalopha troglodyta Larvae[J].Scientia Silvae Sinicae,2007,43(5):59-63. |
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| Authors: | Guo Tongbin Ji Baozhong Zhuge Qiang Huang Minren |
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| Institution: | 1. Forest Pest Management and Quarantine Station of Xuzhou Xuzhou 221004 ; 2. College of Forest Resources and Environment, Nanjing Forestry University Nanjing 210037 |
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| Abstract: | The larvae of Micromelalopha troglodyta were fed with leaves of transgenic poplars expressing Bt gene only and expressing Bt and CpTI genes, the activities of esterase in the midgut of larvae raised in the earlier stage, but restricted later. When the larvae were treated with leaves of transgenic poplars expressing Bt alone and expressing both genes respectively, the corresponding highest restrictive stage was 48, 12 h, and the activities decreased by 15.8%, 38.1%. The restrictive efficiency of the clone expressing both Bt and CpTI was superior to that expressing Bt alone. The restriction of the poplars with Bt gene to carboxylesterase in the midgut of larvae was not notable, but the plants with Bt and CpTI genes could restrain the activities of the Carboxyleterase significantly. When the larvae were fed with leaves of transgenic poplars expressing both genes for 24, 36, 48 h, the activities of carboxyleterase decreased by 33.4%, 22.5%, 29.6% respectively, which had a significant difference with CK. So it was shown that the transgenic poplars disturbed the insect regular metabolism mainly by restraining the activities of esterase and carboxyleterase in the midgut of larvae, then killed the pests. |
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| Keywords: | transgenic poplars Micromelalopha troglodyta detoxification enzymes esterase carboxylesterase |
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