| 酶标SPA抑制染色法检测貉细小病毒的研究——(Ⅰ)酶标SPA抑制染色法试验程序的确立 |
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| 引用本文： | 许树林,沈秀丽,刘鼎新.酶标SPA抑制染色法检测貉细小病毒的研究——(Ⅰ)酶标SPA抑制染色法试验程序的确立[J].黑龙江畜牧兽医,1995(8). |
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| 作者姓名： | 许树林 沈秀丽 刘鼎新 |
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| 作者单位： | 黑龙江省野生动物研究所，东北农业大学生物工程系，中国农科院哈尔滨兽医研究所 哈尔滨 150040 |
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| 摘 要： | 应用感染貉细小病毒(RPV)的72h猫肾传代细胞(F_(81))制做细胞抗原片。利用超迷离心结合蔗糖密度梯度离心法提纯RPV病毒作为免疫用抗原,制备了豚鼠抗RPV高免血消。在酶标SPA染色法的基础上测得高免血清最佳工作范围为160~×、320~×、640~×;PPA的最佳工作效价为40~×;被检样品与标准阳性血清作用最佳温度为37℃,时间为60min。确立了酶标SPA抑制染色法的试验程序。并对有关试剂保存有效期进行了试验测定。
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| 关 键 词： | 酶标SPA 貉细小病毒 血凝试验 血凝抑制试验 |
Studies on the detection of raccoon dogs Parvovirus by PPA InhibitionStaing I Experimental Serial Methods |
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| Xu Shu Lin Shen Xiuli Liu Dingxin.Studies on the detection of raccoon dogs Parvovirus by PPA InhibitionStaing I Experimental Serial Methods[J].Heilongjiang Animal Science And veterinary Medicine,1995(8). |
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| Authors: | Xu Shu Lin Shen Xiuli Liu Dingxin |
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| Institution: | The Heilongjiang province wildlife research institute Herbin 150040 |
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| Abstract: | In the present paper.the cell antigen slids was prepared Usingfeline Kindey cells infected by raccoon parvorius (RPV)for 72 hours.RPV,grown in feline kindey cells,was parified through the sucrose densitygradient hypervelocity centrifugation. Hypcrimmune sera against RPV Wereobtaincd fron guinea pigs which were inoculated with purified RPVcombined with Freurd adjuvant. PPA inhibition staing was established forthe detection of RPV. |
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| Keywords: | PPA Raccoon dog parvovirus HA HI |
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