| 口服O157:H7(ΔhlyΔstxΔtoxB)能够减少O157:H7在山羊体内的定殖 |
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| 引用本文： | 张雪寒,何孔旺,俞正玉,茅爱华.口服O157:H7(ΔhlyΔstxΔtoxB)能够减少O157:H7在山羊体内的定殖[J].华北农学报,2012(3):123-125. |
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| 作者姓名： | 张雪寒 何孔旺 俞正玉 茅爱华 |
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| 作者单位： | 江苏省农业科学院兽医研究所,农业部兽用生物制品工程技术重点实验室,国家兽用生物制品工程技术研究中心 |
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| 基金项目： | 江苏省自然科学基金(BK2010068);江苏省农业支撑计划-社会发展(BE2011771);国家科技支撑计划(2012BAK08B07) |
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| 摘 要： | 为了明确O157:H7(ΔhlyΔstxΔtoxB)口服能否减少亲本株O157:H7在山羊体内的定殖。选用3月龄山羊,首先口服接种O157:H7(ΔhlyΔstxΔtoxB),分别于第7,14天再次口服接种亲本株O157:H7,监测亲本株O157:H7的排菌持续时间和排菌量。结果表明:O157:H7(ΔhlyΔstxΔtoxB)口服后第7天攻击亲本株O157:H7,第4天检测不到亲本株O157:H7的排菌;第14天攻击亲本株O157:H7,第9天检测不到亲本株O157:H7的排菌。直接口服亲本株O157:H7,排菌一直持续到第25天。口服O157:H7(ΔhlyΔstxΔtoxB)能够减少O157:H7在山羊体内的定殖,为研制EHECO157:H7基因缺失疫苗奠定了基础。
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| 关 键 词： | O157:H7(ΔhlyΔstxΔtoxB) 山羊 排菌 |
Oral Inoculation with O157:H7(ΔhlyΔstxΔtoxB) Leads to Decreased Shedding in Goats after Challenge with Escherichia coli O157:H7 |
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| ZHANG Xue-han,HE Kong-wang,YU Zheng-yu,MAO Ai-hua.Oral Inoculation with O157:H7(ΔhlyΔstxΔtoxB) Leads to Decreased Shedding in Goats after Challenge with Escherichia coli O157:H7[J].Acta Agriculturae Boreali-Sinica,2012(3):123-125. |
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| Authors: | ZHANG Xue-han HE Kong-wang YU Zheng-yu MAO Ai-hua |
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| Institution: | (Institute of Veterinary Medicine,Jiangsu Academy of Agricultural Sciences,Key Laboratory of Engineering Research of Veterinary Bio-products of Agricultural Ministry,National Center for Engineering Research of Veterinary Bio-products,Nanjing 210014,China) |
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| Abstract: | Clarify whether oral inoculation with O157:H7(ΔhlyΔstxΔtoxB) leads to decreased shedding in goats after challenge with Escherichia coli O157:H7.Three months old goats were chosen to orally inoculate EHEC O157:H7(ΔhlyΔstxΔtoxB)at 0 day and inoculate parent EHEC O157:H7 at 7 days and 14 days,respectively.EHEC O157:H7 of shedding in feces were detected by plate cultivation.The goats challenged with EHEC O157:H7 at 7 days did not shed bacteria in their feces on the 4th day,and the goats at 14 days no shedding on the 9th day,whereas the goats only with EHEC O157:H7 were shedding bacteria for 23 days.Oral inoculation with O157:H7(ΔhlyΔstxΔtoxB) was able to decrease shedding and colonization EHEC O157:H7 in goats.Therefore,our study provides data to develop attenuated vaccine of EHEC O157:H7. |
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| Keywords: | O157:H7(ΔhlyΔstxΔtoxB) Goats Shedding bacteria |
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