| 重铬酸钾对泥鳅鳍细胞系的毒理学研究 |
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| 引用本文： | 吴迪,周诗珈,李霞,秦艳杰,白丽雯,王文文.重铬酸钾对泥鳅鳍细胞系的毒理学研究[J].大连海洋大学学报,2016(6):646-650. |
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| 作者姓名： | 吴迪 周诗珈 李霞 秦艳杰 白丽雯 王文文 |
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| 作者单位： | 1. 大连海洋大学辽宁省海洋生物资源恢复与生境修复重点实验室,辽宁大连,116023;2. 大连海洋大学辽宁省海洋生物资源恢复与生境修复重点实验室,辽宁大连116023;大连海洋大学农业部北方海水增养殖重点实验室,辽宁大连116023 |
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| 基金项目： | 国家自然科学基金资助项目(31272650) |
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| 摘 要： | 为探究重铬酸钾的毒性机制,建立适合监测铬污染的体外检测系统,以体外培养的泥鳅Misgurnus anguillicaudatus鳍细胞系(DIMF)为试验材料,研究了重铬酸钾的毒性效应。结果表明:通过噻唑蓝(MTT)比色法测定重铬酸钾染毒后细胞的24 h半致死浓度为(25.3±1.2)μmol/L;暴露在浓度为0~30μmol/L的重铬酸钾中,细胞超氧化物歧化酶(SOD)活性随着染毒浓度的增加而增大;谷胱甘肽超氧化物酶(GSH-Px)在重铬酸钾浓度为0~20μmol/L时活性升高,当染毒浓度为30 mol/L时,活力开始下降;谷胱甘肽S-转移酶(GST)活性则随重铬酸钾浓度的升高而降低;微核试验显示,DIMF微核率随染毒浓度的增加呈先升高后降低的趋势,其中最大微核率为0.733%;实时定量PCR结果显示,对照组的金属硫蛋白(MT)基因表达量很低,经重铬酸钾诱导后MT基因表达量显著升高(P0.01)。研究表明,重铬酸钾可对细胞的酶系统和遗传物质造成一定的损伤。
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| 关 键 词： | 泥鳅 重铬酸钾 细胞系 酶活力 微核率 金属硫蛋白 |
Cytotoxicity of potassium dichromate to loach fin cell lines in vitro |
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| Abstract: | Loach Misgurnus anguillicaudatus fin cell line (DIMF) was used to assess toxicity of potassium dichromate in vitro to study toxicity mechanism of potassium dichromate and establish suitable for chrome pollution detection system.The results showed that the median lethal concentration (LCs0) was (25.3 ± 1.2) μmol/L in DIMF exposed to potassium dichromate for 24 h measured by thiazole blue (MTT) method.The activities of superoxide dismutase (SOD) in DIMF were increased with the increase in concentration within potassium dichromate concentration of 0-30 μmol/L and the activities of glutathione peroxidase (GSH-Px) were increased at 0-20 μmol/L potassium dichromate,and then decreased at concentration of 30 μmol/L,with gradual decrease in glutathione Stransferase(GST) activity.Micronucleus test revealed that the rate of micronucleus was increased first and then decreased with the increase in potassium dichromate concentration,with the maximum micronucleus rate of 0.733％.Real-time PCR showed that metallothionein (MT) gene expression quantity was very low in control group,and increased significantly due to the induction by potassium dichromate(P＜0.01).The findings indicate that potassium bichromate can damage enzyme system and genetic materials in cells. |
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| Keywords: | Misgurnus anguillicaudatus potassium dichromate cell line enzyme activity micronucleus rate metallothionein |
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