| Folin-Ciocaileu 比色法测定新疆不同产地紫花苜蓿中总多酚含量 |
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| 引用本文： | 燕雪花,刘宏炳,孙芸,田树革,雷海民.Folin-Ciocaileu 比色法测定新疆不同产地紫花苜蓿中总多酚含量[J].安徽农业科学,2012,40(32):15724-15725. |
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| 作者姓名： | 燕雪花 刘宏炳 孙芸 田树革 雷海民 |
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| 作者单位： | 新疆名医名方与特色方剂学重点实验室,新疆乌鲁木齐830011;新疆医科大学中医学院,新疆乌鲁木齐830011;北京中医药大学中药学院,北京,100029 |
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| 基金项目： | 新疆医科大学科研创新基金项目，新疆名医名方与特色方剂学实验室开放项目 |
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| 摘 要： | 目的]测定新疆不同产地紫花苜蓿中总多酚的含量。方法]以没食子酸作为对照品,以福林试剂-碳酸钠溶液体系为显色剂,采用Folin-Ciocaileu比色法在检测波长760 nm处测定紫花苜蓿中总多酚含量。结果]紫花苜蓿中总多酚含量的标准曲线的回归方程为Y=91x+0.011 8(r=0.999 5),表明在0～6 mg/ml浓度范围内浓度与吸光度的线性关系良好,平均回收率分别为102.34%、103.94%、102.81%。结论]该方法简便、快速、准确,是检测新疆不同产地紫花苜蓿总多酚成分的可靠方法。
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| 关 键 词： | 新疆紫花苜蓿 总多酚 Folin-Ciocaileu 比色法 |
Determination of the Content of Total Polyphenol for Medicago sativa L.of Xinjiang by Folin-Ciocaileu |
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| Abstract: | Objective] To establish an UV method for determination of the content of total polyphenol for M.sativa of Xinjiang.Method] With gallic acid as CK,Folin-Ciocaileu was adopted determining the content of total polyphenol by UV at 760 nm.Results] The regressive equation of standard curve is Y=91x+0.011 8(r=0.999 5).It was indicated that in the range of 0-6 mg/ml,the liner relationship between concentration and absorbance is good.The average recovery was 102.34%,103.94%,102.81%.Conclusion] The method is simple,rapid and accurate,which is a reliable method for detecting total polyphenol from M.sativa in Xinjiang. |
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| Keywords: | Medicago sativa of Xinjiang Total polyphenol Folin-Ciocaileu colorimetry |
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