| 红天麻箭麻发育进程中天麻素和多糖含量的变化规律研究 |
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| 引用本文： | 舒伟,张兴国,程方叙,陈雷,杨言琛.红天麻箭麻发育进程中天麻素和多糖含量的变化规律研究[J].安徽农业科学,2012,40(32):15649-15650,15662. |
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| 作者姓名： | 舒伟 张兴国 程方叙 陈雷 杨言琛 |
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| 作者单位： | 西南交通大学生命科学与工程学院,四川成都,610031;西南交通大学生命科学与工程学院,四川成都,610031;西南交通大学生命科学与工程学院,四川成都,610031;西南交通大学生命科学与工程学院,四川成都,610031;西南交通大学生命科学与工程学院,四川成都,610031 |
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| 基金项目： | 四川省"十二五"天麻育种科技支撑计划项目 |
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| 摘 要： | 目的]研究红天麻箭麻在不同发育阶段的天麻素和天麻多糖含量的相关性规律,确定其品质特征,为红天麻GAP质量控制提供科学依据。方法]在川天麻主产区采集传统主流红天麻品系箭麻块茎样品,在原产地室内采用原位动态沙藏法贮藏箭麻;在采样后沙藏10、80、95、175和220 d分别采集天麻块茎样品,采用高效液相色谱法和苯酚-硫酸比色法分别测定天麻素含量和天麻多糖含量,并运用SPSS17.0版统计软件分析在红天麻箭麻不同发育阶段进程中天麻素和天麻多糖含量的相关性规律。结果]川产红天麻箭麻块茎的天麻素和天麻多糖的平均含量分别为0.36%和8.17%;随着天麻沙藏休眠期-萌动期-抽茎开花-果实成熟至腐烂发育阶段进程增加,红天麻箭麻块茎的天麻素和天麻多糖含量下降,且呈极显著的负相关(P<0.001),其回归方程分别为:天麻素y1=-2E-08x3-1E-06x2-0.0001x+0.365(R2=0.996),天麻多糖y2=-5E-07x3-1E-06x2-0.008x+8.267(R2=0.996)。结论]红天麻箭麻品质较优良,其块茎沙藏发育进程和品质下降密切相关,开花后空心和腐烂的红天麻块茎不宜作药用和制药原料。
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| 关 键 词： | 红天麻(Gastrodia elata Bl.f.elata) 箭麻 发育进程 天麻素 天麻多糖 相关回归分析 |
The Experimental Study on the Developmental Processes and the Relevance of the Contents of Gastrodin and Gastrodia Polysaccharides of Gastrodia elata Bl.f.elata's Sprouted Tuber |
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| Institution: | SHU Wei et al(Life Science and Engineering College,Southwest Jiaotong University,Chengdu,Sichuan 610031) |
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| Abstract: | Objective] By studying the relevance on the contents of gastrodin and gastrodia polysaccharides from Gastrodia elata Bl.f.elata's sprouted tubers in varied developmental stages,determine its quality characteristics,and provide a scientific basis for quality control of red Gastrodia GAP.Method] The sprouted tuber samples from traditional mainstream Gastrodia elata Bl.f.elata in the main producing areas of the Sichuan Gastrodia and that stored in situ dynamic sand in contury of origins indoor were collected,then stored in sand 10 d,80 d,95 d,175 d,220 d.Measuring the content of gastrodin by HPLC and the content of gastrodia polysaccharides by phenol-sulfuric acid method;Using the SPSS17.0 statistical software to study the relevance on the content of gastrodin and gastrodia polysaccharides of Gastrodia elata Bl.f.elata in varied developmental stages and processes.Result] The average contents of gastrodin and gastrodia polysaccharides of Sichuan Gastrodia elata Bl.f.elata during the sprouting period were 0.36% and 8.17%,which increased from the sand period of dormancy,germination period,pumping stem and flowering period,fruiting period to the decay,the content of gastrodin and gastrodia polysaccharides declined highly and showed as negative correlation,the regression equations are: gastrodin y1=-2E-08x3-1E-06x2-0.000 1x+0.365(R2=0.996***),gastrodia polysaccharides y2=-5E-07x3-1E-06x2-0.008x+8.267(R2=0.996***).Conclusion] The quality of Gastrodia elata Bl.f.elata's spourted tuber is excellent,whose decline is closely related to the developmental process in sand,so the hollow and rotten Gastrodia elata Bl.f.elata tubers after flowering are not suitable for medicinal and pharmaceutical raw materials. |
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| Keywords: | Gastrodia elata Bl f elata Spourted tuber Developmental processes Gastrodin Gastrodia polysaccharides Relevant regression analysis |
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