| 甘薯辐射和组培相结合筛选突变体 |
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| 引用本文： | 陆漱韵,濮绍京.甘薯辐射和组培相结合筛选突变体[J].作物学报,1993,19(4):309-314. |
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| 作者姓名： | 陆漱韵 濮绍京 |
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| 作者单位： | 北京农业大学，北京，100094 |
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| 摘 要： | 本研究采用副照甘薯强奸民意苗，接种单节段，离体培养并筛选变异体。以三种不同基因型作副射材料。初步明确了辐射和组培结合过程中一些基本技术条件，如培养基、基因型、光照条件、组培条件下的辐照剂量等，初步获得薯皮色变异系，对线虫性糠腐病抗扩展的变异系。它比常规辐射育种可节省大量田间工作量，并能缩短育种击期，
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| 关 键 词： | 辐射和组培相结合，单节段，腋芽 |
| 收稿时间： | 1991-11-23 |
| 修稿时间： | 1992-02-13 |
Study on Using Gamma Irradiation Combined with in Vitro Culture to Select Mutant in Sweet Potato |
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| Lu Shu-yun,Pu Shao-jin,Wang Jia-xu Beijing Agricultural University,Beijing.Study on Using Gamma Irradiation Combined with in Vitro Culture to Select Mutant in Sweet Potato[J].Acta Agronomica Sinica,1993,19(4):309-314. |
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| Authors: | Lu Shu-yun Pu Shao-jin Wang Jia-xu Beijing Agricultural University Beijing |
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| Institution: | Beijing Agricultural University,Beijing,100094 |
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| Abstract: | Cuttings from 3 different genotypes of sweet potato were irradiated, cut into singlenode and cultured on specific culture medium in order to select expected mutants.Some foundamental problems about the tissue culture of irradiated sweet potato werepreliminarily solved, including the suitable genotype, culture medium, light and tempera-ture conditions and dosage of irradiation. A mutant line of skin color of tuber rootwas obtained and so was a variant resistant to nematode. Through this approach alot of field labour could be saved and the breeding Cycle shorten, It was also foundthat the tube cultured new plantlet did not grow directly from the axillary bud of theirradiated cuttings in the tube; the bud usually grow for a certain period and thenstopped for 1--2 weeks; a new axillary bud would be differentiated and a newplantlet would grow from the new bud. |
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| Keywords: | Irradiation In vitro culture Single node Axillary bud |
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