Androgenic capability among genotypes of winter and spring barley |
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| Authors: | Katarzyna Makowska Sylwia Oleszczuk Aleksandra Zimny Andrzej Czaplicki Janusz Zimny |
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| Institution: | Plant Breeding and Acclimatization Institute ‐ National Research Institute (IHAR‐PIB), Radzikow, Blonie, Poland |
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| Abstract: | Variable efficiency of androgenesis remains a serious problem in many species of cereals. It is still unclear what makes certain genotypes more amenable to androgenesis than others. This study was undertaken to quantify the previously suspected advantage of winter barley genotypes over spring ones with regard to regeneration efficiency in anther culture. The material consisted of 40 barley hybrids originating from Polish breeding companies. The number of androgenic structures per 100 anthers did not differ significantly between analysed groups (119 vs. 152 non‐significant), but the average regeneration of green plants per 100 anthers was five times higher in winter genotypes (6.4 vs. 1.3). The incidence of albinism was lower for the winter than for the spring materials (70% vs. 90%), while the rate of spontaneous chromosome doubling was similar in both groups (58% vs. 56%). The results strongly support the notion that winter genotypes are more amenable to androgenesis and this may be a consequence of their better adaptation to stress conditions. |
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| Keywords: | androgenesis albinism anther culture winter spring doubled haploid |
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