| 福寿螺18S rRNA和28S rRNA基因片段的克隆与进化分析 |
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| 引用本文： | 潘颖瑛,董胜张,俞晓平.福寿螺18S rRNA和28S rRNA基因片段的克隆与进化分析[J].植物保护,2009,35(1):33-36. |
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| 作者姓名： | 潘颖瑛 董胜张 俞晓平 |
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| 作者单位： | 1. 杭州师范大学生命和环境科学学院,杭州,310036
2. 中国计量学院生命科学学院,杭州,310018 |
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| 基金项目： | 浙江省科技厅重大项目 |
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| 摘 要： | 为从分子水平上明确入侵我国的福寿螺在分类学上的地位，采用分子克隆和序列比对的方法，对来自菲律宾及我国广东、广西、浙江等不同地理种群福寿螺的18S rRNA基因和28S rRNA基因片段进行扩增、克隆和序列测定，并同瓶螺科、田螺科和环口螺科相关物种进行系统发育分析。结果表明，获得的福寿螺18S rRNA基因和28S rRNA基因片段长度分别为602 bp、325 bp，且不同地理种群间碱基序列无差异。通过邻接法（NJ）和最大简约法（MP）构建的系统树基本一致，证实福寿螺隶属于瓶螺科，与田螺科物种亲缘关系较近，而与环口螺科亲缘关系较远。
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| 关 键 词： | 福寿螺 18S rRNA基因 28S rRNA基因 系统进化分析 |
Cloning and phylogenetic analysis of 18S rRNA and 28S rRNA genes of Pomacea canaliculata |
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| Pan Yingying,Dong Shengzhang,Yu Xiaoping.Cloning and phylogenetic analysis of 18S rRNA and 28S rRNA genes of Pomacea canaliculata[J].Plant Protection,2009,35(1):33-36. |
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| Authors: | Pan Yingying Dong Shengzhang Yu Xiaoping |
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| Institution: | 1. College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou310036, China；2. College of Life Sciences, China Jiliang University, Hangzhou310018, China |
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| Abstract: | 18S rRNA and 28S rRNA genes in different geographic populations of Pomacea canaliculata, including Philippines, Guangdong, Guangxi, Zhejiang in China, were cloned and sequenced. The phylogenetic relationships between the families Ampullariidae, Viviparidae and Cyclophoridae were analyzed. The results showed that 18S rRNA and 28S rRNA genes were 602 bp and 325 bp in length, respectively. The phylogenetic trees based on NJ (Neighbor Joining) and MP (Maximum Parsimony) were almost identical. It is shown that P. canaliculata belongs to Ampullariidae, which has a closer relationship with Viviparidae than with Cyclophoridae. |
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| Keywords: | Pomacea canaliculata 18S rRNA gene 28S rRNA gene phylogenetic analysis |
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