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| 自发性急性犬瘟热的原发性脱髓性脑病 | |
| 引用本文： | 潘耀谦,龙塔,赵德明,Seiichi HIGUCHI.自发性急性犬瘟热的原发性脱髓性脑病[J].中国兽医学报,2005,25(3):225-230,262. |
| 作者姓名： | 潘耀谦 龙塔 赵德明 Seiichi HIGUCHI |
| 作者单位： | 1. 河南科技大学,动物科技学院,河南,洛阳,471003;中国农业大学,动物医学院,北京,100094 2. 河南科技大学,动物科技学院,河南,洛阳,471003 3. 中国农业大学,动物医学院,北京,100094 4. 日本北里大学 动物畜产学部内科学教研室,日本,十和田 034-8628 |
| 摘 要： | 为了进一步观察犬瘟热病毒引起的原发性脑损伤和包涵体形成的特点，调查脑组织的损伤与神经症状的关系，对10只急性犬瘟热病犬的脑组织进行了详细的病理学研究。为了仔细地观察病变，本试验按照解剖学关系将脑组织分成3个大部分和11个切面，即大脑(4个切面)，脑干(5个切面)和小脑(2个切面)。组织切片经HE、LFB和免疫组织化学染色后进行检查，结果表明：在大脑，脱髓呈弥漫性发生，程度较轻；脑干的周围或靠近第三脑室的白质脱髓较重；小脑在轻度或中度脱髓的基础上常出现严重的多发性脱髓灶。脱髓部呈空泡或海绵状，有少量胶质细胞存在，但无炎性反应。脱髓性病损是非时称性发生，对神经束没有特殊的亲和力。在脑室的室管膜细胞内发现有较多的嗜酸性胞浆或核内包涵体。用抗犬瘟热病毒抗体染色，带有包涵体的室管膜细胞呈现强阳性反应。部分锥体细胞，神经核细胞和漓氏细胞变性、溶解或胞浆深染。胞核浓缩。这种变化以小锥体神经细胞表现得最为明显。根据此研究结果，作者认为由犬瘟热病毒引起的原发性脑组织损伤是一种脱髓性脑病，而不是脑炎变化；位于室管膜细胞内的包涵体对于脑组织犬瘟热的确诊具有重要的作用；由于犬瘟热病毒引起神经细胞的损伤是非特异性的，对脑组织的侵害是非对称性的。对神经束的作用无特殊的亲和力，所以患犬瘟热的犬在临床上可出现不同的神经症状。 |
| 关 键 词： | 原发性 脑病 急性 自发性 免疫组织化学染色 犬瘟热病毒 神经症状 神经细胞 脑组织 核内包涵体 组织切片 第三脑室 胶质细胞 病毒抗体 阳性反应 锥体细胞 研究结果 组织损伤 非特异性 非对称性 亲和力 细胞内 室管膜 脑损伤 |
Primary Demyelinating Encephalopathy in Dogs with Spontaneous Acute Canine Distemper |
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| PAN Yao-qian,LONG Ta,ZHAO De-ming,Seiichi HIGUCHI.Primary Demyelinating Encephalopathy in Dogs with Spontaneous Acute Canine Distemper[J].Chinese Journal of Veterinary Science,2005,25(3):225-230,262. | |
| Authors: | PAN Yao-qian LONG Ta ZHAO De-ming Seiichi HIGUCHI |
| Institution: | PAN Yao-qian~1,2,LONG Ta1,ZHAO De-ming2,HIGUCHI Seiichi3 |
| Abstract: | Pathological characterizations of the central nervous system (CNS) of dogs with acute canine distemper were the demyelination in white matter and the formation of inclusion body in astrocytes. In order to further observe the features of primary brain lesions and inclusion body induced by Canine distemper virus (CDV) and investigate the reliant clinical neurological signs on brain lesions 10 dogs with acute canine distemper were detected in detail. To detect the lesions carefully the brain tissue was divided into three portions and eleven slices, that is cerebrum (five slices), cerebral stem (four slices) and cerebellum (two slices) according to anatomical location; stained with hematoxylin and eosin (HE), luxol fast blue (LFB) and Immunohistochemical assay. The results revealed that the specific demyelination and mild lesions was widespread in cerebra tissue and severe demyelination was encircling or near the third ventricle in the brain stem. Base on mild or moderate diffuse demyelination the severe multifoci lesions occurred in cerebella. In the demyelinating areas vacuolation and spongy appearance were obvious, glial cells were a few and no inflammatory reactions were displayed. The demyelinating lesions were non-symmetric appearance and no special affinity for particular traces. The more eosinophilic intracytoplasmic and intranuclear inclusion bodies were found in ependymal cells of lateral and the third ventricle and astrocytes that were often near the ventricles. With anti-CDV antigen assay, the ependymal cells with inclusion bodies showed an intensive positive reaction. Some of pyramidal cells, nervous nucleus cells and Purkinje cells were degeneration and cytolysis, or shrunken with pyknotic nucleus, especially in small pyramidal cells. According to this experiment it was considered that the primary brain lesions induced by CDV were a demyelinating encephalopathy, but not encephalitis. The inclusion bodies located in ependymal cells were a important evidence for determination of canine distemper in brain tissues. The brain lesions induced by CDV were non-specific injury about neuron and nervous nuclei, so that manifested the different neutrological signs. |
| Keywords: | canine distemper demyelination encephalopathy neutrological signs inclusion body |
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