| 基于20种桉树叶绿体matK基因序列的系统发育分析 |
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| 引用本文： | 刘果,;谢耀坚,;张党权,;罗建中,;曾艳飞.基于20种桉树叶绿体matK基因序列的系统发育分析[J].桉树科技,2014(3):1-9. |
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| 作者姓名： | 刘果 ;谢耀坚 ;张党权 ;罗建中 ;曾艳飞 |
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| 作者单位： | [1]国家林业局桉树研究开发中心,广东湛江524022; [2]中南林业科技大学林业生物技术湖南省重点实验室经济林育种与栽培国家林业局重点实验室经济林培育与保护省部共建教育部重点实验室,湖南长沙410004; [3]中国林业科学研究院林业研究所国家林业局林木培育重点实验室,北京100091 |
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| 基金项目： | “十二五”农村领域国家科技计划课题“白桦、桉树等分子育种与品种创制”（2011AA100202） |
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| 摘 要： | 随着公共数据库信息量的不断丰富,桉树基因组序列的公布数据也逐渐增多。本文利用NCBI数据库下载的21条桉树叶绿体matK基因序列进行分析研究,发现软件构建的系统发育树树与学术界承认和使用的Hill＆Johnson形态分类学状态有一定的差异,可能与下载的序列数据不够全面、覆盖的信息量不能代表该种基因组序列有关。通过对MP法、ML法、NJ法、UPGMA法和Bayes法等5种方法构建系统发育树、序列信息位点分析和形态学分类状态的比较,UPGMA法构建的系统发育树与序列的信息位点分析结果较为一致,MP法构建的系统发育树较为可信,但进一步的信息确认还需后续的研究证实。
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| 关 键 词： | 桉树 叶绿体 matK基因 系统发育分析 |
Phylogenetics of 20 Species of Eucalyptus Based on Sequences of the Chloroplast matK Gene |
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| Institution: | LIU Guo, XIE Yao-jian, ZHANG Dang-quan, LUO Jian-zhong, ZENG Yan-fei (1. China Eucalypt Research Centre, Zhanjiang 524022, Guangdong, China; 2. Central South University of Forestry and Technology; Hunan Provincial Key Laboratory of Forestry Biotecknology ; Key Laboratory of Non-wood Forest Products of State Forestry Ministry; Key Laboratory of Non-wood Forest Trees and Protection of Ministry of Education, Changsha 410004, Hunan, China; 3 Research Institute of Forestry, Chinese Academy of Forestry; Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration, Beijing 100091, China) |
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| Abstract: | With the enrichment of public databases, the release of Eucalyptus genome sequence data has gradually been increasing. In this study, 21 Eucalyptus chloroplast matK gene sequences were downloaded from the NCBI database for analytical use. Depending on the software used, we found the phylogenetic trees obtained could differ from the morphological taxonomic status according to Hill Johnson, which is accepted as the academic standard. These differences might relate to the sequence data not being comprehensive, as the amount of information obtained can＇t represent the entire genome sequence. When phylogenetic trees constructed from the MP method, the ML method, the NJ method, the UPGMA method and the Bayes method were compared to the results of sequence information site analysis and the morphological classification status, the results showed that the phylogenetic tree constructed from UPGMA method was consistent with the sequence information site analysis results, and the phylogenetic tree constructed from MP method was more credible. However, further information needed to confirm these results will require follow-up studies. |
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| Keywords: | Eucalyptus chloroplast matK gene phylogenetic analysis |
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