| 4株淡水微藻的适宜温度、光照强度及其细胞组成 |
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| 引用本文： | 曾蓓蓓,黄旭雄,危立坤,刘林林.4株淡水微藻的适宜温度、光照强度及其细胞组成[J].上海海洋大学学报,2014,23(6):856-862. |
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| 作者姓名： | 曾蓓蓓 黄旭雄 危立坤 刘林林 |
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| 作者单位： | 上海海洋大学,上海海洋大学 |
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| 基金项目： | 国家海洋局项目（SHME2011SW02），上海高校水产学一流学科建设项目资助 |
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| 摘 要： | 采用单因子试验方法,研究了不同温度和光照强度对4株微藻(多芒藻Golenkinia sp.SHOU-F56,绿球藻Chlorococcum sp.SHOU-F93,月牙藻Selenastrum sp.SHOUF119和绿球藻Chlorococcum sp.SHOU-F124)生长的影响,并分析了在适宜培养温度和光照条件下4株微藻细胞的生化组成。结果表明:多芒藻,绿球藻SHOU-F93,月牙藻和绿球藻SHOU-F124的最适温度条件分别为29.5、19.3、21.5和24.3℃;最适光照强度分别为35.5、8.3、22.5和19.2μmol/(m2·s)。绿球藻SHOU-F93的色素和总糖含量最高,分别占细胞干重的3.26%和20.90%,蛋白质较高的为绿球藻SHOU-F93和月牙藻,分别为32.64%和33.91%,月牙藻的总脂含量最高(23.74%)。4株微藻主要的脂肪酸为16∶0、18∶1n-9、18∶2n-6和18∶3n-3。依据4株微藻的细胞组成特点,后续可作为淡水饵料微藻开发利用。
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| 关 键 词： | 微藻 生长 温度 光照强度 细胞组成 |
| 收稿时间： | 4/1/2014 12:00:00 AM |
| 修稿时间： | 6/5/2014 12:00:00 AM |
The suitable culture temperature, light intensity for four freshwater microalgae and analysis on cellular biochemical compositions |
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| ZENG Bei-bei,HUANG Xu-xiong,WEI Li-kun and LIU Lin-lin.The suitable culture temperature, light intensity for four freshwater microalgae and analysis on cellular biochemical compositions[J].Journal of Shanghai Ocean University,2014,23(6):856-862. |
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| Authors: | ZENG Bei-bei HUANG Xu-xiong WEI Li-kun and LIU Lin-lin |
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| Institution: | Shanghai Ocean University,Shanghai Ocean University |
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| Abstract: | In this study,a culture experiment was conducted to study the effects of different temperature and light intensity on the growth of four species of microalgae（ Golenkinia sp. SHOU-F56,Chlorococcum sp.SHOU-F93, Selenastrum sp. SHOU-F119, Chlorococcum sp. SHOU-F124）, and the biochemical compositions and fatty acids of the microalgae were analyzed. The results showed that the optimal culture temperatures were 29. 5,19. 3,21. 5,24. 3 ℃ and the optimal light intensity was 35. 5,8. 3,22. 5,19. 2μmol /（ m2·s） for Golenkinia sp. SHOU-F56,Chlorococcum sp. SHOU-F93,Selenastrum sp. SHOU-F119 and Chlorococcum sp. SHOU-F124,respectively. Under the suitable culture conditions,Chlorococcum sp.SHOU-F93 cells had the highest contents of pigments（ 3. 26%） and carbohydrates（ 20. 90%）,while Chlorococcum sp. SHOU-F93 and Selenastrum sp. SHOU-F119 had the highest contents of protein,being32. 64% and 33. 91%,respectively and Selenastrum sp. SHOU-F119 had the highest contents of total lipid（ 23. 74%）. The dominant fatty acids in the four microalgae were 16∶ 0,18∶ 1n-9,18∶ 2n-6 and 18∶ 3n-3.Based on characteristics of four microalgae cells,these microalgae may have propects for the development and utilization as freshwater bait. |
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| Keywords: | microalgae temperature light intensity biochemical composition |
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