Antigenic differences between H5N1 human influenza viruses isolated in 1997 and 2003 |
| |
| Authors: | Horimoto Taisuke Fukuda Nao Iwatsuki-Horimoto Kiyoko Guan Yi Lim Wilina Peiris Malik Sugii Shunji Odagiri Takato Tashiro Masato Kawaoka Yoshihiro |
| |
| Institution: | Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, The University of Tokyo, Japan. |
| |
| Abstract: | To assess whether the antigenic properties of H5 hemagglutinin (HA) change over time due to antigenic drift, we produced a panel of monoclonal antibodies (mAbs) against the HA of the index H5N1 human influenza A virus, A/Hong Kong/156/97. By immunizing mice with a plasmid expressing this HA and boosting the initial immunization with cell lysates transfected with the plasmid, a total of six hybridomas producing HA-specific mAbs were established: four to the HA1 subunit with hemadsorption-inhibiting activity and two to the HA2 subunit. None of the mAbs to HA1 could bind to the HA of a recent human isolate, A/Hong Kong/213/2003, indicating that there are substantial antigenic differences between the H5N1 human influenza virus isolated in 1997 and that isolated in 2003. |
| |
| Keywords: | |
| 本文献已被 PubMed 等数据库收录！ |
|
