| 非洲菊花瓣的离体培养 |
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| 引用本文： | 刘国华,陈海燕,宋刚,张虎.非洲菊花瓣的离体培养[J].安徽农业科学,2004,32(2):316-317. |
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| 作者姓名： | 刘国华 陈海燕 宋刚 张虎 |
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| 作者单位： | 江苏农林职业技术学院,江苏句容,212400 |
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| 摘 要： | 试验以不同花色非洲菊的花瓣为材料 ,在添加不同浓度的 2 ,4-D和 6-BA的培养基上进行愈伤组织诱导和芽的分化。结果表明 :不同花色愈伤组织的诱导率存在差别 ;不同浓度的 2 ,4-D和BA对愈伤组织的诱导和分化的效果明显不同 ,2 ,4-D和BA浓度过高对诱导和分化均有抑制作用 ;诱导花瓣形成愈伤组织的最佳培养基为MS +2 ,4-D 2 .0mg/L +NAA 0 .2mg/L +BA0 .1mg/L ,花瓣不定芽分化的最佳培养基为MS +BA 8.0mg/L +NAA 0 .1mg/L。
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| 关 键 词： | 非洲菊 2 4-D BA 愈伤诱导 分化 |
| 文章编号： | 0517-6611(2004)02-0316-02 |
Tissue Culture of Gerbera Jamesonii |
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| Liu Guohua et al.Tissue Culture of Gerbera Jamesonii[J].Journal of Anhui Agricultural Sciences,2004,32(2):316-317. |
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| Authors: | Liu Guohua |
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| Abstract: | The experiment was conducted to study the different color of Gerbera jamesonii and different combination of 2,4-D or BA in MS medium. The results indicated that different color had significant effect on callus induction, concentration of 2,4-D decided the number of calli, the highest frequency(74%) of callus induction of petal were obtained in MS containing 2,4-D 2?mg/L.Too high concentration of 2.4-D or BA had negative effect.The best differentiation medium for petal was MS+BA?8.0?mg/L+NAA?0.1?mg/L. |
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| Keywords: | Gerbera jamesonii 2 4-D BA Callus induction Differentiation |
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