| 鸡新城疫流行毒株与标准毒株的交叉免疫保护试验 |
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| 引用本文： | 刘燕,荀来武,杨晓花,何华川.鸡新城疫流行毒株与标准毒株的交叉免疫保护试验[J].中国畜牧兽医,2007,34(2):102-104. |
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| 作者姓名： | 刘燕 荀来武 杨晓花 何华川 |
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| 作者单位： | 1．云南农业职业技术学院，昆明 650212；2.云南省家畜冷冻精液站，昆明 650212 |
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| 摘 要： | 用新城疫(ND)分离强毒油佐剂灭活苗和NDV La Sota油佐剂灭活苗分别免疫母源抗体平均为2 log2的12日龄公雏。免疫后每隔1周采集血清，用微量血凝抑制试验检测HI抗体。免疫后21 d抗体水平达到峰值，抗体水平在6log2以上，维持30 d以上。免疫40 d后，用ND分离强毒株和NDVF48E9分别进行攻毒试验，2种疫苗的保护率均为100％，攻毒结果表明，ND分离强毒株和NDVF48E9株的免疫原性差异不显著。
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| 关 键 词： | 新城疫流行毒株 新城疫标准毒株 免疫保护 |
| 文章编号： | 1671-7236（2007）02-0102-02 |
| 修稿时间： | 2006-08-17 |
A Cross Immunoprophylaxis Experiment with Epidemic Avian NDV Strain and Avian NDV Strain |
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| LIU Yan,XUN Lai-wu,YANG Xiao-hua,HE Hua-chuan.A Cross Immunoprophylaxis Experiment with Epidemic Avian NDV Strain and Avian NDV Strain[J].China Animal Husbandry & Veterinary Medicine,2007,34(2):102-104. |
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| Authors: | LIU Yan XUN Lai-wu YANG Xiao-hua HE Hua-chuan |
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| Institution: | 1．Yunnan Agricultural Vocational technical College，Kunming 650212，China； 2.Yunnan Domestic Animal Frozen Semen Station，Kunming 650212，China |
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| Abstract: | 12 day old chicken,which averange titres of NDV antibodies is 2log2,was immunized with the oil adjuvant NDV velogenic strains vaccine and the oil adjuvant NDV La Sota strains vaccine.After the inoculability, the serum was pooled every week,and the titres of NDV antibodies was showed by the Micro-HIA.The peak titres of NDV antibodies was appeared the 21th day,and the 6log2 high titres was keeping above 30 days.40 days later in the immunization,the chicken were challenged by NDV velogenic isolates and NDVF48E9 isolates,and no chicken was infected.The percentage of protection from the two vaccines are 100%.The test of challege indicated that immunigenicity difference between the oil adjuvant NDV velogenic strains vaccine and the oil adjuvant NDV LaSda strains vaccine is not remarkable. |
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| Keywords: | epidemic NDV strain NDV strain immunoprophylaxis |
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