Cod CGRP and tachykinins in coeliac artery innervation of the Atlantic cod, <Emphasis Type="Italic">Gadus morhua</Emphasis>: presence and vasoactivity |
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| Authors: | Fatemeh Shahbazi Susanne Holmgren Jörgen Jensen |
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| Institution: | 1.Department of Zoophysiology,G?teborg University,Goteborg,Sweden |
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| Abstract: | The presence and vasoactive effects of native calcitonin gene-related peptide (CGRP), substance P (SP), and neurokinin A (NKA)
were studied on isolated small branches of the coeliac artery from Atlantic cod, Gadus morhua, using immunohistochemistry and myograph recordings, respectively. Immunohistochemistry revealed nerve fibers containing
CGRP- and SP/NKA-like material running along the wall of the arteries. CGRP induced vasorelaxation of precontracted arteries
with a pD2 value of 8.54 ± 0.17. Relaxation to CGRP (10−8 M) was unaffected by l-NAME (3 × 10−4 M) and indomethacin (10−6 M) suggesting no involvement of nitric oxide or prostaglandins in the CGRP-induced relaxation. SP and NKA (from 10−10 to 3 × 10−7 M) contracted the unstimulated arteries at concentrations from 10−8 M and above in 42% and 33%, respectively, of the vessels. It is concluded that the innervation of the cod celiac artery includes
nerves expressing CGRP-like and tachykinin-like material, and that a vasodilatory response to CGRP is highly conserved amongst
vertebrates while the response to tachykinins is more variable. |
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| Keywords: | Immunohistochemistry In vitro pharmacology NKA Substance P Teleost fish |
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