| 两种LD50计算方法对副溶血性弧菌毒力的比较研究 |
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| 引用本文： | 董雪红,田敏,季策,马文元,张庆华.两种LD50计算方法对副溶血性弧菌毒力的比较研究[J].上海海洋大学学报,2016,25(1):86-96. |
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| 作者姓名： | 董雪红 田敏 季策 马文元 张庆华 |
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| 作者单位： | 上海海洋大学 水产种质资源发掘与利用教育部重点实验室,上海海洋大学 水产种质资源发掘与利用教育部重点实验室,上海海洋大学 水产种质资源发掘与利用教育部重点实验室,上海海洋大学 水产种质资源发掘与利用教育部重点实验室,上海海洋大学 水产种质资源发掘与利用教育部重点实验室 |
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| 基金项目： | 上海市教育委员会科研创新项目（13ZZ127），教育部留学回国人员科研启动基金资助项目，上海海洋大学博士启动基金项目（A-0209-13-0105344），水产动物遗传育种中心上海市协同创新中心项目（ZF1206） |
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| 摘 要： | 副溶血性弧菌(Vibrio parahaemolyticus,Vp)是目前备受关注的人畜共患病的病原。为了比较7株不同来源的Vp对斑马鱼的毒力大小及致病能力,采用急性毒性试验测定96 h内Vp对斑马鱼的半数致死剂量(median lethal dosage,LD_(50)),用累积法和Bliss法对比分析LD_(50)指标。结果显示:Vp KNH1无致病性。其他菌株感染后,均表现出腹部出血,腹腔有腹水,内脏出血等症状。用累积法和Bliss法分析人类致病菌株ATCC17802、ATCC33847及水生动物致病菌株Vp13、Vp31、Vp41、Vp57共6株不同来源的副溶血性弧菌的LD_(50),累积法计算得到的LD_(50)分别为6.0×107、7.4×107、3.9×107、1.6×108、1.6×108、9.3×107CFU/m L;Bliss法得到的LD_(50)分别为:5.0×107、7.6×107、3.6×107、1.5×108、1.6×108、9.4×107CFU/m L。研究结果发现两种计算方法得出的LD_(50)数值差距较小,结果相似,不同菌株毒力强弱的顺序均为:Vp13ATCC17802ATCC33847Vp57Vp31≥Vp41Vp KNH1。综上所述,累积法及Bliss法均可用于检测细菌毒力大小,但由于计算机的发展使得Bliss计算方法更加简便,为更优的选择。
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| 关 键 词： | 副溶血弧菌 斑马鱼 LD50 累积法 Bliss法 |
| 收稿时间： | 5/4/2015 12:00:00 AM |
| 修稿时间： | 2015/7/31 0:00:00 |
The comparative study on the LD50 to evaluate the virulence of Vibrio parahaemolyticus using two calculation methods |
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| DONG Xuehong,TIAN Min,JI Ce,MA Wenyuan and ZHANG Qinghua.The comparative study on the LD50 to evaluate the virulence of Vibrio parahaemolyticus using two calculation methods[J].Journal of Shanghai Ocean University,2016,25(1):86-96. |
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| Authors: | DONG Xuehong TIAN Min JI Ce MA Wenyuan and ZHANG Qinghua |
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| Institution: | Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources,Ministry of Education,Shanghai Ocean University,Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources,Ministry of Education,Shanghai Ocean University,Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources,Ministry of Education,Shanghai Ocean University,Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources,Ministry of Education,Shanghai Ocean University,Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources,Ministry of Education,Shanghai Ocean University |
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| Abstract: | The Vibrio parahaemolyticus(Vp) is paid much attention to as a zoonosis pathogen at present.In order to compare the virulence and pathogenicity of seven Vp strains from different sources, we used zebrafish as the model to test median lethal dosage (LD50)by acute toxicity experiment within 96h, and analyzed the data with ReeD-Muench method and Bliss method.The results suggested that the LD50 tested by ReeD-Muench method of human pathogenic bacteria (ATCC17802, ATCC33847) and aquatic animal pathogens(Vp13, Vp31, Vp41, Vp57)were 6.0×107, 7.4×107, 3.9×107, 1.6×108, 1.6×108, 9.3×107CFU/mL, respectively and 5.0×107,7.6×107,3.6×107,1.5×108,1.6×108,9.4×107 CFU/mL, respectively, with Bliss method. The results showed that these two kinds of calculation methods had little difference for the LD50, and the virulence of these seven different Vp strains were as follows: Vp13 > ATCC17802 > ATCC33847 > Vp57 > Vp31 > Vp41 > VpKNH1.To conclude, ReeD-Muench method and Bliss method can both be used to test Vp strains virulence.However, Bliss calculation method is more simple and accurate than the ReeD-Muench method with the development of computer technology.As a result, it is the better choice for LD50. |
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| Keywords: | Vibrio parahaemolyticus zebrafish LD50 ReeD-Muench method Bliss method |
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