| 基于高通量测序技术的酿酒葡萄产区土壤微生物多样性 |
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| 引用本文： | 王伟,布丽根·加冷别克,胡晓东,夏俊芳,张志东,顾美英,武运.基于高通量测序技术的酿酒葡萄产区土壤微生物多样性[J].新疆农业科学,2020,57(5):859-868. |
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| 作者姓名： | 王伟 布丽根·加冷别克 胡晓东 夏俊芳 张志东 顾美英 武运 |
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| 作者单位： | 1.新疆农业大学食品科学与药学学院,乌鲁木齐 830052;2.新疆农业科学院微生物应用研究所,乌鲁木齐 830091 |
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| 基金项目： | 新疆维吾尔自治区重大科技专项(2017A01001-2-3) |
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| 摘 要： | 【目的】研究新疆4大酿酒葡萄产区土壤中的微生物群落结构及多样性,为构建新疆酿酒葡萄主产区的微生物菌库奠定基础。【方法】分别从新疆酿酒葡萄主产区采集土壤样品,提取总DNA,通过Illumina MiSeq高通量测序结合生物信息学分析,得到新疆酿酒葡萄主产区的土壤微生物群落结构及多样性。【结果】在12份土壤样品中,伊犁河谷土壤中细菌的群落多样性最高,天山北麓土壤中细菌群落多样性最低;吐哈盆地中真菌群落多样性最低,焉耆盆地中真菌的群落多样性最高。节杆菌属细菌和耐冷酵母属真菌是吐哈盆地和天山北麓葡萄园土壤中的优势菌属;芽单胞菌属细菌和刚毛四枝孢属真菌是伊犁河谷葡萄园土壤中的优势菌属;节杆菌属细菌和赤霉菌属真菌是焉耆盆地葡萄园土壤中的优势菌属。土壤pH值对细菌群落组成的影响最显著,Mn元素含量对真菌群落组成的影响最显著。【结论】揭示了新疆酿酒葡萄主产区土壤中细菌和真菌群落组成,新疆酿酒葡萄产区的微生物多样性存在差异。
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| 关 键 词： | Illumina MiSeq高通量测序 酿酒葡萄 土壤 微生物群落 多样性 |
| 收稿时间： | 2019-09-17 |
Analysis of the Microbial Community Diversity of Soil from Wine Grape Productiing Area in Xinjiang Based on High-throughput Sequencing |
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| WANG Wei,Buligen Jialengbieke,HU Xiaodong,XIA Junfang,ZHANG Zhidong,GU Meiying,WU Yun.Analysis of the Microbial Community Diversity of Soil from Wine Grape Productiing Area in Xinjiang Based on High-throughput Sequencing[J].Xinjiang Agricultural Sciences,2020,57(5):859-868. |
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| Authors: | WANG Wei Buligen Jialengbieke HU Xiaodong XIA Junfang ZHANG Zhidong GU Meiying WU Yun |
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| Institution: | 1.College of Food Sciences and Pharmacy, Xinjiang Agricultural University, Urumqi 830052, China;2.Research Institute of Applied Microbiology, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China |
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| Abstract: | 【Objective】 To find out the microbial community structure and diversity in the soil of the four major wine grape producing areas in Xinjiang and to lay a foundation for constructing the microbial bank in the main wine grape producing areas in the region. 【Methods】 After total DNA was extracted, Illumina MiSeq high-throughput sequencing system and bioinformatics analysis were used for the investigation of microbial communities and species diversity of main wine grape producing area in Xinjiang. 【Results】 In the twelve soil samples, the results showed that the highest microbial diversity of bacteria was the soil in Yili river-valley and the lowest one was in the soil of northern slope of Tianshan Mountain, and the highest microbial diversity of fungus was in the soil of Yanqi basin and the lowest microbial diversity of fungus was the Tuha basin soil. The dominant bacteria and fungus were Arthrobacter and Guehomyces in Tuha basin and the northern slope of Tianshan Mountain wine grape producing area. The dominant bacteria and fungus were Gemmatimonas and Tetracladium in Yili river-valley wine grape producing area. The dominant bacteria and fungus were Arthrobacter and Gibberella in Yanqi basin wine grape producing area. Soil pH value had the most significant effect on the composition of bacterial community, and the content of Mn had the most significant effect on the composition of fungal community. 【Conclusion】 This study revealed the composition of bacterial and fungal communities in the soil in main wine grape producing areas in Xinjiang, which indicated that there were differences in microbial community diversity. |
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| Keywords: | Illumina MiSeq high-throughput sequencing system wine grapes soil microbial community diversity |
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