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Comparison the Content of Moisture,Ash and Extract from Stem and Root Bark of Eucommia ulmoides Oliver and Euonymus grandiflorus Wall. f. grandiflorus
Abstract:Objective]To compare the content of moisture,ash and extract of stem and root bark of Eucommia ulmoides Oliver and Euonymus grandiflorus Wall.f.grandiflorus.Methods]According to the first method of aquametry(over-drying method)in the 2010 edition Chinese Pharmacopoeia appendix IX H,ash determination in appendix IX K,and extract measurement in appendix X A.Results]Moisture content of stem and root bark of Euonymus grandiflorus Wall.f.grandiflorus was between 5.38%and 6.33%,while that of Eucommia ulmoides Oliver was between 5.36%and 6.90%(both in line with the stipulation in Chinese Pharmacopoeia that moisture content of Eucommia ulmoides Oliver herbs should at most be 13.0%);total ash content of stem and root bark of Euonymus grandiflorus Wall.f.grandiflorus was between15.88%to 19.43%,while that of Eucommia ulmoides Oliver was between 5.45%and 7.30%(The average content of Eucommia ulmoides Oliver was 6.29%,and it was in line with the stipulation in Chinese Pharmacopoeia that of total ash content of Eucommia ulmoides Oliver should at most be 10%;and the average content of stem and root bark of Euonymus grandiflorus Wall.f.grandiflorus was 17.36%,and it was higher than the stipulated level.);the acid-insoluble ash content of stem and root bark of Euonymus grandiflorus Wall.f.grandiflorus was between 1.88%and 2.64%,lower than that of Eucommia ulmoides Oliver(3.58%-4.10%);the content of 75%hot ethanol extract of Euonymus grandiflorus Wall.f.grandiflorus was the highest of 26.51%;and under the extract condition,the content of stem and root bark of Euonymus grandiflorus Wall.f.grandiflorus was between 19.36%and 26.45%,obviously higher than that of Eucommia ulmoides Oliver(14.96%-16.70%)(Both of that were in line with the stipulation in Chinese Pharmacopoeia,and the ethanol-soluble extract content of stem and root bark of Eucommia ulmoides Oliver should be at least 12.0%);there was little difference in ether volatile extract content of Euonymus grandiflorus Wall.f.grandiflorus and Eucommia ulmoides Oliver,respectively in the range of 0.33%-0.51%and 0.33%-0.56%.Conclusions]The appropriate moisture content of Euonymus grandiflorus Wall.f.grandiflorus should not exceed 13%.Euonymus grandiflorus Wall.f.grandiflorus are widely used by of Yi nationality clinic,however it was few research on chemical components,bioactivity and the action mechanism of toxicology and pharmacology.Moreover results of this experiment showed that the content of ethanol-soluble extract of Euonymus grandiflorus Wall.f.grandiflorus was higher than that of Eucommia ulmoides Oliver by hot 75%ethanol extraction,while content of ether volatile extract in the two materials were similar.Therefore,bio-screening of main constituents and relevant researches on effects of toxicology and pharmacology should be done to testify and ensure the safety and validity of Yi Nationality clinic,developed and medical used scientifically and properly.
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