| Abstract: | Objective]To explore the methods for extraction and content determination of polysaccharides from Hedysayum polybotrys Hand.-Mazz.,and to observe its anti-inflammatory effect. Methods] Polysaccharides were extracted by hot water. The content determination was performed at wavelength 490 nm with phenol-sulfuric acid as a chromomeric agent. Results]The content of HPS was 4. 94% in same batch of radix hedysari slices,while that reached 45. 30% in refined radix hedysari samples. HPS inhibited the xylene-induced ear swelling in mice.Conclusions]The phenol-sulfuric acid method is simple,rapid,and accurate,and HPS has anti-inflammatory effect. |
