Effect of Manually Preheparinized Syringes on Packed Cell Volume and Total Solids in Blood Samples Collected from American Alligators (Alligator mississippiensis) |
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| Institution: | 1. “Epidermis Differentiation and Rheumatoid Autoimmunity” Laboratory, UMR CNRS 5165, INSERM U1056, Toulouse III University, Toulouse, France;2. Laboratory of Cell Biology and Cytology, Toulouse University Hospital, Toulouse, France;3. Unit of Rheumatology, School of Medicine, University of Yaounde I, Cameroon;4. Arthritis Research UK Center for Genetics and Genomics, Centre for Musculoskeletal Research, Manchester Academic Health Science Centre, The University of Manchester, UK;5. Division of Rheumatology, University Hospitals of Geneva, Geneva, Switzerland;6. Department of Genetics and Laboratory Medicine, Geneva, Switzerland;7. Department of Pathology & Immunology, University of Geneva, School of Medicine, Geneva, Switzerland;1. School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, SP, Brazil;2. Anesthesiology Laboratory (LIM08), School of Medicine, University of São Paulo, São Paulo, SP, Brazil;1. Ophthalmology section, Negah Veterinary Center, Tehran, Iran;2. Department of Clinical Sciences, Faculty of Specialized Veterinary Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran;3. Department of Clinical Sciences, College of Veterinary Medicine, Karaj Branch, Islamic Azad University, Alborz, Iran |
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| Abstract: | The hemodilution effect of manually preheparinized syringes was determined by evaluating the packed cell volume (PCV) and total solids (TS) from blood samples collected from 50 American alligators (Alligator mississippiensis). A volume of 0.2 mL of blood was drawn into 3 syringes of 1 mL: control with no heparin sodium, heparin sodium drawn to 0.1 mL and then expelled, and heparin sodium drawn to 0.2 mL and then expelled. PCV and TS values were determined from each syringe immediately after collection. Mean PCV and TS values were compared between the control and heparin groups using linear mixed modeling. The syringes coated with heparin resulted in a significantly lower mean PCV and TS values when compared with controls, with no significant difference between the heparin groups. This dilution effect was also found to be inconsistent and not accurate from one syringe to another. An adjunct method of obtaining 0.5 mL from already-collected blood samples into 1-mL syringes that were coated with heparin drawn to 0.2 mL and then expelled also showed a significant decrease in PCV and TS values when compared with the control samples, although to a lesser extent. As a result, it cannot be recommended to manually preheparinize syringes when collecting small volume of blood samples from American alligators because significant and unpredictable hemodilution is likely to occur. |
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| Keywords: | American alligator heparin packed cell volume syringe total solids |
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