Effects of Water Decoction of Plumbago zeylanica L. on the Proliferation,Apoptosis and Cell Cycle of Rats Hepatic Stellate Cells |
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| Abstract: | Objective] To observe the effects of water decoction of Plumbago zeylanica Linn.on the proliferation,apoptosis and cell cycle of rats hepatic stellate cells,and to discuss the function and mechanism of anti-hepatic fibrosis of P.zeylanica.Methods] SD rats were given water decoction of P.zeylanica by gavage,so as to obtain medicated serum.Medicated serum was incubated together with hepatic stellate cells( HSC-T6) according to different dosage groups.Blank control group was desgined; and medicated serum groups of colchicine and Compound Biejiaruangan Tablet were taken as the positive control groups.Proliferation of HSC-T6 was detected after incubation by MTT colorimetry; cell apoptosis and the DNA content in each cell phase was detected by flow cytometer.Results]Inhibitory rate and apoptosis rate: compared with blank control,inhibitory rate and apoptosis rate in medicated serum groups enhanced significantly,showing significant differences( P 0.01).When the dosage of medicated serum was within 5%- 20%,inhibitory rate and apoptosis rate of HSC-T6 enhanced as dosage increased.Inhibitory rate and apoptosis rate in medicated serum group of high dosage were significantly higher than those in colchicine groups,but was equal to those in Compound Biejiaruangan Tablet group.Cell cycle: there were no significant changes in cell percentage in each group at G2/M phase.Compared with blank control group,cell percentages of medicated serum groups of P.zeylanica enhanced significantly at G0/G1 phase,and reduced significantly at S phase,showing significant changes( P 0.01).When the dosage of medicated serum reduced within 20%- 5%,the cell percentage gradually declined at G0/G1 phase,and gradually increased at S phase.Under the same serum concentration,cell percentage of P.zeylanica group enhanced significantly at G0/G1 phase and decreased obviously at S phase compared with that of colchicine group.There were no significant changes in Compound Biejiaruangan Tablet group.Conclusions] Medicated serum of P.zeylanica could restrict G1/S proliferation and induce its apoptosis,showing a dose-dependent manner.Medicated serum group of P.zeylanica had stronger effects than medicated serum group of colchicine,while had equal effects to the group of Compound Biejiaruangan Tablet.The mechanism of medicated serum of P.zeylanica in inhibiting HSC-T6 proliferation was to block cell cycle at G0/G1 phase,and to prevent it from passing G1/S. |
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