| 芝麻空间诱变品系（种）的DNA指纹分析 |
| |
| 引用本文： | 孙建,涂玉琴,张秀荣.芝麻空间诱变品系（种）的DNA指纹分析[J].中国农业科学,2007,40(12):2696-2701. |
| |
| 作者姓名： | 孙建 涂玉琴 张秀荣 |
| |
| 作者单位： | 1. 中国农业科学院油料作物研究所,武汉,430062
2. 华中农业大学作物遗传改良国家重点实验室,武汉,430070 |
| |
| 基金项目： | 国家高技术研究发展计划(863计划);国家航天育种工程资助项目 |
| |
| 摘 要： | 【目的】揭示芝麻空间诱变品系（种）的DNA水平上的变异，探讨芝麻空间诱变的分子机理，以期为作物空间诱变育种提供理论基础。【方法】首次应用AFLP标记对芝麻空间诱变的3个品系（种）及原始对照种豫芝4号（未搭载处理的留地种子）进行DNA指纹分析。【结果】随机组合的30对AFLP引物对原始对照种及3个空间诱变品系（种）进行扩增，共获得1176条DNA谱带，其中多态性谱带625条，多态性位点比率为53.15%，每对引物均扩增出多态性，并且每一个空间诱变品系（种）与原始对照种间均扩增出多态性DNA谱带。利用NTSYS软件进行类平均法（UPGMA）聚类发现3个空间诱变品系（种）明显地聚在一起，与原始对照种存在较大差异。【结论】通过空间诱变能够在DNA水平上导致芝麻遗传物质变异，空间诱变是一种有效的育种途径。AFLP是一种研究植物空间诱变材料的高效率的分子生物学方法。
|
| 关 键 词： | 芝麻 空间诱变 AFLP DNA指纹 |
| 收稿时间： | 2006-11-13 |
| 修稿时间： | 2006年11月20 |
DNA Fingerprint Analysis in Space-induced Sesame Mutant Lines |
| |
| SUN Jian,TU Yu-qin,ZHANG Xiu-rong.DNA Fingerprint Analysis in Space-induced Sesame Mutant Lines[J].Scientia Agricultura Sinica,2007,40(12):2696-2701. |
| |
| Authors: | SUN Jian TU Yu-qin ZHANG Xiu-rong |
| |
| Abstract: | Abstract：【OBJECTIVE】To reveal differences of sesame space mutantlines in DNA level and discuss their molecular mechanisms which provide theory basis for crop space-flight mutation breeding.【METHOD】DNA fingerprint analysis was performed between three mutantlines and their original CK line “Yuzgi No.4” by amplified fragments length polymorphism (AFLP) technology for the first time.【RESULTS】Three mutantlines and their CK line were analysed by random selecting 30 pairs AFLP primers, 625 polymorphic bands were produced in 1176 DNA bands which were obtained in four samples, and its degree of gene polymorphism was 53.15%. DNA polymorphic bands were amplified between three mutantlines and their CK line during every primer pairs. UPGMA clustering showed that three mutantlines which were similar had biggish differences by comparing with “Yuzhi No.4”. 【CONCLUSSION】Space-flight mutagenesis could result in variation of sesame germ plasm, so space-flight mutantion breeding was an effective approach of plant breeding. AFLP was also a high efficient molecular biological method of researching plant space-flight mutagenesis. |
| |
| Keywords: | AFLP |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《中国农业科学》浏览原始摘要信息 |
| 点击此处可从《中国农业科学》下载免费的PDF全文 |
