| 兔脂肪间充质干细胞分离培养及鉴定 |
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| 引用本文： | 刘玉梅,张自强,杨雪峰,邓雯,李梦云.兔脂肪间充质干细胞分离培养及鉴定[J].西北农业学报,2009,18(5):43-47. |
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| 作者姓名： | 刘玉梅 张自强 杨雪峰 邓雯 李梦云 |
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| 作者单位： | 1. 河南科技大学,动物科技学院,河南洛阳,471003
2. 河南科技学院,动物科技学院,河南新乡,453003 |
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| 摘 要： | 为了建立一种体外培养兔脂肪间充质干细胞(ADSCs)的方法,在无菌条件下取家兔双侧腹股沟脂肪垫,Ⅰ型胶原酶消化后,用含10%胎牛血清的DMEM 培养液培养.细胞连续传代后,MTT法检测第3、5、10代细胞的增殖情况.采用油红染色法和Von Kossa染色法观察第3代ADSCs向成脂和成骨方向分化情况,并采用流式细胞仪分析细胞表面标志.结果显示,第3、5、10代的ADSCs生长曲线并无显著差异,细胞增殖较快;ADSCs成脂诱导后油红染色阳性,成骨诱导后Von kossa染色形成典型的黑色钙化结节.ADSCs 表面标志检测结果为:CD29 和CD90阳性,CD45和CD80阴性.成功分离和培养了兔的ADSCs,其具有成脂和成骨潜能,可作为组织工程的种子细胞.
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| 关 键 词： | 兔 脂肪间充质干细胞 分离 培养 鉴定 |
| 收稿时间： | 2009/2/23 0:00:00 |
| 修稿时间： | 2009/4/21 0:00:00 |
Isolation, Culture and Characterization of Rabbit Adipose-derived Stromal Cells |
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| LIU Yumei,ZHANG Ziqiang,YANG Xuefeng,DENG Wen and LI Mengyun.Isolation, Culture and Characterization of Rabbit Adipose-derived Stromal Cells[J].Acta Agriculturae Boreali-occidentalis Sinica,2009,18(5):43-47. |
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| Authors: | LIU Yumei ZHANG Ziqiang YANG Xuefeng DENG Wen and LI Mengyun |
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| Institution: | College of Animal Science and Technology, He''nan University of Science and Technology, Luoyang He''nan 471003 China;College of Animal Science and Technology, He''nan University of Science and Technology, Luoyang He''nan 471003 China;College of Animal Science, He''nan Institute of Science and Technology, Xinxiang He''nan 453003 China;College of Animal Science and Technology, He''nan University of Science and Technology, Luoyang He''nan 471003 China;College of Animal Science and Technology, He''nan University of Science and Technology, Luoyang He''nan 471003 China |
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| Abstract: | In order to establish a method for isolation and culture of rabbit adipose-derived stromal cells in vitro and explore their biological characteristics.Inguinal fat pads of rabbit were aseptically separated,followed by digestion with Itype collagenase,then cultured in DMEM with 10% fetal bovine serum and assay its growth curve of the third,fifth and tenth generation.To verify the multipotential differentiation of mesenchymal characteristics of ADSCs,the third passage cells were subjected to differentiation in conditions known to induce adipogenic and osteogenic lineages and used for phenotypic analysis by flow cytometry.There were no significant deviation between growth curve of the third,fifth and tenth passage.Cells had a strong capability of proliferation.After cultured in adipogenic differentiation medium,the cells were Oil Red O staining positive.After cultured in osteogenic differentiation medium,the cells were Von kossa staining positive.The cultured ADSCs in this study were positive for CD29,CD90 and negative for CD80 and CD45.ADSCs were successfully isolated and cultured from rabbit adipose tissue.These cells have adipogenic and osteo genic potential.This study showed that the ADSCs could be served as seed cells in tissue engineering. |
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| Keywords: | Rabbit Adipose-derived stem cells Tissue engineering |
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