| 兰属品种间遗传多样性的AFLP分析 |
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| 引用本文： | 王晓英,王长宪.兰属品种间遗传多样性的AFLP分析[J].农学学报,2014,4(10):69-72. |
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| 作者姓名： | 王晓英 王长宪 |
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| 作者单位： | 泰安市泰山林业科学研究院,山东泰安,271000 |
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| 基金项目： | 2012年泰安市科技发展计划项目“名品春兰的遗传多样性研究”，林业公益性行业科研专项“国兰新品种培育与产业化生产关键技术研究” |
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| 摘 要： | 为从分子水平上研究兰属植物的遗传多样性和建立适合的分子标记反应体系.本研究采用AFLP分子标记技术,对兰属植物的9个品种进行了遗传多样性分析.结果表明,筛选得到的8对引物共扩增出965条DNA片段,其中多态性条带为931条,平均1对引物扩增条带121条（多态性带116条）,多态性位点频率为95.87％,说明遗传多样性丰富.用NTSYS2＆#183;10进行UPGMA聚类分析,属于同种的品种聚在一起,与表型分类基本吻合.兰属植物间遗传相似系数变化范围为0.416-0.606.此分子标记技术体系可为兰属分类及种质遗传多样性分析提供技术依据.
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| 关 键 词： | 兰属 品种 遗传多样性 |
Genetic Diversity Analysis of Cybidium Cultivars by AFLP Molecular Markers |
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| Wang Xiaoying,Wang Changxian.Genetic Diversity Analysis of Cybidium Cultivars by AFLP Molecular Markers[J].Journal of Agriculture,2014,4(10):69-72. |
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| Authors: | Wang Xiaoying Wang Changxian |
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| Institution: | (Taishan Institute of Forestry Science, Tai＇ an 271000, Shandong, China) |
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| Abstract: | The aim was to analyze genetic diversity of Cybidium and establish suitable reaction system from the molecular level. The genetic diversity and relationship of 9 cuhivars of Cymbidium were evaluated using AFLP molecular markers in this study. Among the 965 AFLP bands obtained from eight selective primer pairs, 931 （95.87%） were polymorphic. The average number of DNA bands per primer pair was 121, and the average number of DNA polymorphic bands per primer pair was 116. Using NTSYS2~ 10 to UPGMA cluster analysis, cuhivars originated from the same species could be clustered together in the AFLP dendrogram and this division was in consistence with the morphological classification. Genetic similarity among the cultivars ranged from 0.416 to 0.606. The reaction system of AFLP could be used to the classifying of Cybidium and the analysis of its germplasm genetic diversity. |
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| Keywords: | Cybidium Cuhivar AFLP Genetic Diversity |
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