| 东北马鹿Myostatin基因编码序列的克隆及序列分析 |
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| 引用本文： | 巴恒星,邢秀梅,李春义,杨福合.东北马鹿Myostatin基因编码序列的克隆及序列分析[J].吉林农业大学学报,2010,32(2):200-204. |
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| 作者姓名： | 巴恒星 邢秀梅 李春义 杨福合 |
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| 作者单位： | 中国农业科学院特产研究所,吉林,132109 |
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| 基金项目： | 公益性行业(农业)科研专项基金项目(200903014) |
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| 摘 要： | 根据Myostatin基因的保守性,选择牛(Bos taurus cattle登录号:AB076403)的Myostatin序列为模板进行引物设计,首次从东北马鹿基因组中扩增出Myostatin序列,扩增产物连接pMD18-T载体,克隆出3个外显子片断。根据5-′GU-AG-3′规则和同源基因比对拼接出完整的编码序列,全长为1 128 bp(发表到GenBank登录号:EF629535),Myostatin蛋白前体由375个氨基酸组成。同源比较结果表明:Myostatin在进化过程中具有高度保守性,东北马鹿与猪Myostatin编码序列同源性最高达到98%,鸟类与哺乳动物间Myostatin编码序列同源性为85%~89%,鱼类与其他动物间编码序列同源性为60%~67%。Myostatin编码序列能够真实反映远缘物种的进化关系,可以作为远缘物种进化分析较理想的标记。
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| 关 键 词： | 东北马鹿 Myostatin 基因克隆 同源性分析 |
| 收稿时间： | 2009-04-08 |
| 修稿时间： | 2009-07-29 |
Cloning and Sequence Analysis of Exon of Dongbei Wapiti Myostatin Gene |
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| BA Heng-xing,XING Xiu-mei,LI Chun-yi,YANG Fu-he.Cloning and Sequence Analysis of Exon of Dongbei Wapiti Myostatin Gene[J].Journal of Jilin Agricultural University,2010,32(2):200-204. |
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| Authors: | BA Heng-xing XING Xiu-mei LI Chun-yi YANG Fu-he |
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| Institution: | Institute of Special Economic Animal and Plant, Chinese Academy of AgriculturalSciences, Jilin 132109|China |
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| Abstract: | Three exon fragments of the Myostatin sequence of Dongbei wapiti were cloned from the latest species of cattle (Bos taurus cattle accession: AB076403) for the templates,with primer′s design according to the conservative of Myostatin sequence.Dongbei wapiti Myostatin was amplified for the first time.The products of PCR were linked into pMD18-T vector and then three exons were cloned.Myostatin code sequence was spliced according to 5′-GU-AG-3′ rules and homologous matching.The Myostatin gene code sequence was composed of 1 128 bp (Dongbei wapiti accession: EF629535) coding 375 amino acids.The results showed that the homology analysis of the Myostatin in the evolution was highly conservative.The homology of Dongbei wapiti Myostatin amino acid sequence with pig was up to 98%;the homology of Myostatin amino acid sequence was 85%-89% between birds and mammals;the homology of Myostatin amino acid sequence was 60%-67% between fish and other animals.Myostatin code sequence could really attest to the evolution of distant species,as a better Phylogenetic mark. |
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| Keywords: | Dongbei wapiti Myostatin gene cloning homology analysis |
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