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| 广玉兰叶片抗氧化活性评价 | |
| 引用本文： | 何开跃,李晓储,樊亚苏,张双全,毕慧敏.广玉兰叶片抗氧化活性评价[J].林业科学研究,2007,20(5):644-649. |
| 作者姓名： | 何开跃 李晓储 樊亚苏 张双全 毕慧敏 |
| 作者单位： | 1. 南京林业大学森林资源与环境学院,江苏,南京,210037 2. 江苏省林业科学院,江苏,南京,211153 3. 南京师范大学生命科学学院,江苏,南京,210097 |
| 基金项目： | 南京林业大学校科研和教改项目 |
| 摘 要： | 用乙酸乙酯从广玉兰(Magnolia grandiflora)叶片中萃取出的活性物质,在进行主要成分理化检识鉴定后,以人工抗氧化剂PG(没食子酸丙酯)和天然抗氧化剂PA(植酸)为对照,测定了其体外抗氧化活性,并用两种体系DPPH·(二苯代苦味酰基自由基)和卵磷脂进行评价.结果表明,提取物含有黄酮类化合物和酚类化合物.提取物稀释10倍(92.00 mg·mL-1)时,对过氧化氢的清除率达最大,为29.41%,在稀释20倍(46.00 mg·mL-1)时,抑制活性氧活性达最大,达500活力单位,稀释50倍(18.40 mg·mL-1)时,非酶体系的总抗氧化能力达最大,为270活力单位.DPPH·体系和卵磷脂体系评价结果表明,在提取物稀释50倍时(18.40 mg·mL-1),对DPPH·的清除率为92.69%,比PG强,对脂质过氧化的抑制作用达最大,达56.67%,与PA相当,因此,抗氧化效果最好. |
| 关 键 词： | 广玉兰 DPPH· 卵磷脂 抗氧化 黄酮类化合物 酚类化合物 |
| 文章编号： | 1001-1498（2007）05-0644-06 |
| 收稿时间： | 2007/1/22 0:00:00 |
| 修稿时间： | 2007年1月22日 |
Appraisal of Antiox idation Activity ofMagnolia guandif lora Leaves |
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| HE Kai-yue,LI Xiao-chu,FAN Ya-su,ZHANG Shuang-quan and BI Hui-min.Appraisal of Antiox idation Activity ofMagnolia guandif lora Leaves[J].Forest Research,2007,20(5):644-649. | |
| Authors: | HE Kai-yue LI Xiao-chu FAN Ya-su ZHANG Shuang-quan and BI Hui-min |
| Institution: | Nanjing Forestry University, Nanjing?? 210037, Jiangsu, China;J iangsu A cademy of Forest ry, N an jing?? 211153,J iangsu, Ch ina;Nanjing Forestry University, Nanjing?? 210037, Jiangsu, China;Nan jing Norma lU n ivers ity, N an jing?? 210097, J iangsu, Ch ina;Nanjing Forestry University, Nanjing?? 210037, Jiangsu, China |
| Abstract: | The extract fromMagnolia grand if lora leavesw as iso lated by ethy l acetate and its main components w ereidentified by physical and chem icalm ethods. Its antiox idat ion activ ity w as detected and eva luated by two system s( DPPH?and lec ith in)w ith con tro ls for syn thetic an tiox idant PG ( propy l gallate) and natural antiox idan t PA ( phy ticacid) . The resu lts show ed that the extract conta ined f lavono ids and pheno lic compounds. W hen the ex tractw as d ilu??ted by 10 tim es( 92. 00mg?mL-1 ), its scaveng ing rate to H2O2 atta ined the h ighest, 29. 41% ; when itw as d ilu??ted by 20 t imes( 46. 00mg?mL-1 ), it had the h ighest activ ity to restra in ing act ive oxygen, 500 un its. The activ ityof tota l antiox idation from non??enzym e system w as 270 activ ity un its when the ex tract w as d iluted by 50 t imes ( 18.40 mg?mL-1 ). The resu lts o f appraisal by DPPH?and lec ithin systems show ed tha twhen the ex tractw as dilutedby 50 times( 18. 40mg?mL-1 ) , it had the best effect o f antiox idation w ith the highest scavenging rate to DPPH ??( 92. 69% ), wh ich w as h igher than PG and the restraining rate to lip id perox idat ion( 56. 67% ) w as as same as PA .Th is result prov ided scient ific basis for further approach ing and explo iting the b iolog ical act iv ity ofM. grand if lora. |
| Keywords: | Magnolia grand if lora DPPH? lecithin ant iox idation flavono ids phenolic compounds |
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