| 绿原酸对猪精液冷冻保存效果的影响 |
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| 引用本文： | 何涛,李青旺,余四九.绿原酸对猪精液冷冻保存效果的影响[J].家畜生态学报,2021,32(1):56-60. |
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| 作者姓名： | 何涛 李青旺 余四九 |
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| 作者单位： | 甘肃农业大学;宁夏职业技术学院;西北农林科技大学 |
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| 摘 要： | 为探究绿原酸对猪精液冷冻保存效果的影响,分别在TCG稀释液中添加不同浓度(15、30、50、80和100μg/mL)的绿原酸,通过测定冷冻-解冻后精子的活率、顶体完整率、质膜完整率、DNA完整率、超氧化歧化酶(SOD)、过氧化氢酶(CAT)和谷胱甘肽过氧化物酶(GSH-Px)活性来判定对保存效果的影响。结果表明,当添加剂量为50μg/mL时,保护效果最好,精子的活率、顶体完整率、质膜完整率、SOD、CAT和GSH-Px活性等参数均高于对照组(P<0.05);在不同处理组中,冻后精子活率和SOD活性在添加剂量为30和50μg/mL时,较其他处理组差异显著(P<0.05);质膜完整率、CAT和GSH-Px活性在添加剂量为15和50μg/mL时,较其他处理组差异显著(P<0.05)。试验结果表明,TCG稀释液中添加50μg/mL绿原酸对猪精液冷冻保存效果最佳。
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| 关 键 词： | 绿原酸 猪精液 冷冻保存 精子质量 |
Effects of Chlorogenic Acid on Cryopreservation of Boar Sperm |
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| HE Tao,LI Qingwang,YU Sijiu.Effects of Chlorogenic Acid on Cryopreservation of Boar Sperm[J].Acta Ecologae Animalis Domastici,2021,32(1):56-60. |
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| Authors: | HE Tao LI Qingwang YU Sijiu |
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| Institution: | (Gansu Agricultural University,Lanzhou Gansu 730070,China;Ningxia Polytechnic,Yinchuan Niangxia 750021,China;Northwest A&F University,Yangling Shaanxi 712100,China) |
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| Abstract: | To explore the effect of chlorogenic acid on the cryopreservation of boar semen,different concentrations(15,30,50,80 and 100μg/ml)of CGA were added to the TCG diluent respectively.The quality of spermatozoa was determined by measuring the activity rate,acrosome integrity rate,plasma membrane integrity,DNA integrity,superoxide dismutase,catalase and glutathione peroxidase activity after freezing-thawing.The results showed that when the concentration of additives was 50μg/ml,the protection effect was the best,the plasma membrane integrity rate,SOD,CAT and GSH-Px activities were significantly higher than those of the control group(P<0.05);in different treatment groups,when the addition dose was 30 and 50μg/ml,the difference was significant compared with other treatment groups(P<0.05);when the addition dose was 15 and 50μg/ml,the difference was significant compared with other treatment groups(P<0.05).The results showed that adding 50μg/ml CGA had the best effect on cryopreservation of boar semen. |
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| Keywords: | chlorogenic acid boar sperm cryopreservation sperm quality |
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