| 多种因素对小鼠卵母细胞体外受精效果影响的分析 |
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| 引用本文： | 张景锋,郝京京,徐秋良,朱宽佑,牛晖,张长兴.多种因素对小鼠卵母细胞体外受精效果影响的分析[J].家畜生态学报,2021,42(3):38-42. |
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| 作者姓名： | 张景锋 郝京京 徐秋良 朱宽佑 牛晖 张长兴 |
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| 作者单位： | 河南牧业经济学院动物科技学院,河南郑州450046;河南省畜禽遗传资源保护工程技术研究中心,河南郑州450046;河南牧业经济学院动物科技学院,河南郑州450046 |
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| 基金项目： | 国家自然科学基金(31601915);河南牧业经济学院博士科研启动基金项目(51000788)。 |
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| 摘 要： | 为了探讨不同精子获能时间,精卵孵育时间,精子密度以及颗粒细胞对小鼠卵母细胞体外受精的影响,从而达到对卵母细胞体外受精体系优化的目的。比较了精子获能时间分别为40 min、60 min、80 min试验组的受精卵卵裂率。结果表明,带颗粒细胞卵母细胞(COCs)在三个试验组中卵裂率无显著差异,不带颗粒细胞卵母细胞(NO)在精子获能时间为60 min时卵裂率最高;比较了精卵孵育时间分别为2 h、4 h、6 h、8 h试验组的受精卵卵裂率,结果显示COCs精卵孵育时间2 h试验组的效果最好,NO孵育时间为6 h试验组的效果最好;比较了精子密度分别为3×105/mL,3×106/mL,3×107/mL试验组受精卵卵裂率,结果显示COCs和NO均为3×106/mL试验组卵裂效果最好;比较COCs和NO的受精卵卵裂率,结果显示COCs与NO之间存在显著差异(P<0.05),裸卵卵裂效果显著优于颗粒细胞卵裂效果。试验结果表明,在卵母细胞体外受精过程中,精子获能时间60 min,精子密度为3×106/mL,精卵孵育6 h,培养24 h后卵裂率最高。
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| 关 键 词： | 卵母细胞 精子获能 精子密度 体外受精 |
Effect of Various Factors on Mouse Oocytes in Vitro Fertilization |
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| ZHANG Jingfeng,HAO Jingjing,XU Qiuliang,ZHU Kuanyou,NIU Hui,ZHANG Changxing.Effect of Various Factors on Mouse Oocytes in Vitro Fertilization[J].Acta Ecologae Animalis Domastici,2021,42(3):38-42. |
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| Authors: | ZHANG Jingfeng HAO Jingjing XU Qiuliang ZHU Kuanyou NIU Hui ZHANG Changxing |
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| Institution: | (College of Animal Science,Henan University of Animal Husbandry and Economy,Zhengzhou,Henan,450046,China;Henan Engineering Technology Research Center of Livestock and Poultry Genetic Resources Protection,Zhengzhou,Henan,450046,China) |
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| Abstract: | To investigate the effects of different sperm capacitation time,sperm incubation time,sperm density and granulosa cells on in vitro fertilization of mouse oocytes,so as to optimize in vitro fertilization system of oocytes,the fertilized ovum cleavage rate of the experimental group at 40 min,60 min and 80 min were compared respectively.The results showed that there was no significant difference in the cleavage rate of COCs in the three groups at the capacitation time,and NO had the highest cleavage rate at the capacitation time of 60 min.The cleavage rate of fertilized eggs in the experimental group with incubation time of 2 h,4 h,6 h and 8 h,were also studied respectively,which showed that the COCs group with incubation time of 2h had the best effect,while the NO group with incubation time of 6h had the best effect.The cleavage rate of fertilized eggs in the experimental group with sperm density being 3×105/mL,3×106/mL and 3×107/mL were compared respectively,showing that both COCs and NO had the best cleavage effect in the 3×106/mL group.Comparing the cleavage rate of fertilized eggs with COCs and NO,the results showed that there was a significant difference between COCs and NO,and the cleavage effect of naked eggs was significantly higher than that of granulosa cells(P<0.05).In the process of in vitro fertilization of oocytes,the sperm capacitation time was 60min,the sperm density was 3×106/mL,the sperm and eggs were incubated for 6 h,and the cleavage rate was the highest after 24 h. |
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| Keywords: | oocyte sperm capacitation sperm density fertilization in vitro |
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