| 利用花粉管通道法将耐草甘膦基因mG2-epsps 导入玉米自交系的研究初报 |
| |
| 引用本文： | 王秀君,郎志宏,陆伟,林敏,单安山,黄大昉.利用花粉管通道法将耐草甘膦基因mG2-epsps 导入玉米自交系的研究初报[J].中国农业科技导报,2008,10(4):56-62. |
| |
| 作者姓名： | 王秀君 郎志宏 陆伟 林敏 单安山 黄大昉 |
| |
| 作者单位： | [1]中国农业科学院生物技术研究所,北京100081 [2]东北农业大学动物科技学院,哈尔滨150030 |
| |
| 基金项目： | 国家自然科学基金，国家高技术研究发展计划(863计划) |
| |
| 摘 要： | 构建了含有耐草甘膦基因mG2-epsps的植物表达载体pUmG2，采用花粉管通道法将其导入优良玉米自交系X90中，对Tn代1542株植株进行草甘膦喷洒实验，共得到32株能够耐受0．25％草甘膦药液的抗性植株；对这32株抗性植株的PCR检测结果表明，其中29株为PCR阳性植株，平均转化率为1．88％；Southern杂交和Western杂交检测结果证明mG2-epspps基因已整合至玉米基因组并正确表达。
|
| 关 键 词： | 花粉管通道法 草甘膦 raG2-epsps基因 转基因玉米 |
| 收稿时间： | 2008-06-02 |
| 修稿时间： | 2008-06-20 |
Transferring Glyphosate-tolerant mG2-epsps Gene into Maize |Inbred Line via Pollen-tube Pathway |
| |
| WANG Xiu-jun,LANG Zhi-hong,LU Wei,LIN Min,SHAN An-shan,HUANG Da-fang.Transferring Glyphosate-tolerant mG2-epsps Gene into Maize |Inbred Line via Pollen-tube Pathway[J].Journal of Agricultural Science and Technology,2008,10(4):56-62. |
| |
| Authors: | WANG Xiu-jun LANG Zhi-hong LU Wei LIN Min SHAN An-shan HUANG Da-fang |
| |
| Institution: | 1. Biotechnology Research Institute, Chinese Academy of Agricultural Science, Beijing 100081 ; |2. College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China |
| |
| Abstract: | A plant expression vector pUmG2 harboring the glyphosate-tolerant mG2-epsps gene was constructed and transferred into the maize inbred line X90 by pollen-tube pathway. Among the 1 542 transformed plants of TO generation, 32 glyphosate-tolerant plants were survived after sprayed with 0.25% glyphosate, and 29 of them were con- firmed positive plants by PCR analysis. The average transformation rate was 1.88%. Southern blot and Western blot showed that the rnG2-epsps gene had been introduced into maize genome and expressed correctly. |
| |
| Keywords: | pollen-tube pathway glyphosate mG2-epsps gene transgenic maize |
| 本文献已被 维普 万方数据 等数据库收录！ |
| 点击此处可从《中国农业科技导报》浏览原始摘要信息 |
| 点击此处可从《中国农业科技导报》下载免费的PDF全文 |
