| 日本百脉根γ-生育酚甲基转移酶基因的电子克隆及进化分析 |
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| 引用本文： | 孟宪萍,李晓晓,李雅轩,蔡民华,胡英考.日本百脉根γ-生育酚甲基转移酶基因的电子克隆及进化分析[J].安徽农业科学,2006,34(24):6457-6459. |
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| 作者姓名： | 孟宪萍 李晓晓 李雅轩 蔡民华 胡英考 |
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| 作者单位： | 首都师范大学生命科学学院,北京,100037 |
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| 基金项目： | 北京市教委科技发展计划项目基金资助(200310028112) |
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| 摘 要： | 根据物种间同源基因相对保守的特点,利用生物信息学方法,以拟南芥-γ生育酚甲基转移酶cDNA序列为信息探针,在GenBank的HTGs数据库中找到与之高度同源的日本百脉根基因组DNA序列——LjT44D21克隆。通过GENSCAN分析及序列拼接得到日本百脉根"γTMT基因的cDNA序列,GenBank的登陆号为DQ013360。用该序列对GenBank中的est-others进行相似性搜索,获得了44条日本百脉根的EST序列,经拼接后发现与基因组预测的基因序列一致,表明该基因是真实存在和表达的。该cDNA序列长为1 077 bp,具有完整的开放阅读框架(1～1 077 bp)。推测编码蛋白为358个氨基酸,该蛋白序列与大豆、水稻、小麦、拟南芥、油菜、苜蓿和衣藻等物种的-γTMT蛋白质序列进行了同源性比较分析,具有很高的同源性。
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| 关 键 词： | 日本百脉根 γ-生育酚甲基转移酶 电子克隆 EST |
| 文章编号： | 0517-6611（2006）24-6457-03 |
| 收稿时间： | 07 10 2006 12:00AM |
| 修稿时间： | 2006-07-10 |
In silico Cloning of Gamma-tocopherol Methyltransferase Gene from Lotus corniculatus var. japonicus |
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| MENG Xian-ging, et al.In silico Cloning of Gamma-tocopherol Methyltransferase Gene from Lotus corniculatus var. japonicus[J].Journal of Anhui Agricultural Sciences,2006,34(24):6457-6459. |
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| Authors: | MENG Xian-ging |
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| Institution: | College of Life Science, Capital Normal University, Beijing 100037 |
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| Abstract: | In silico cloning is a new method for gene cloning.Arabidopsis thialiana γ-TMT cDNA sequence(GenBank accession number AY090280) being used as a querying probe to BLAST the HTGs database,a highly homologous DNA sequence,which was referred as LjT44D21clone,was obtained.After performing the GENSCAN program,a positive gene,which comprised of 6 exons and 5 introns,was found.With the predicted sequence to BLAST GenBank est-others database,44 ESTs homologous sequences hit can be edited to a unique sequence which was the same as the predicted sequence from genomic DNA.These results showed that this gene authentically existed and expressed.This 1 077 bp gene contained a complete ORF(1 bp-1 077 bp),encoded 358 amino acids,which was very conserved with Glycine max,Oryza sative,Triticum aestivum,Arabidopsis thaliana,Brassica oleracea,Medicago truncatula and Chlamydomonas reinhardtii,respectively. |
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| Keywords: | Lotus corniculatus var japonicus γ-TMT in silico cloning EST |
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