| 真菌诱导物对丹参毛状根和转化细胞过氧化物酶活力的影响 |
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| 引用本文： | 黄柏青,刘曼西.真菌诱导物对丹参毛状根和转化细胞过氧化物酶活力的影响[J].湖北农学院学报,2001,21(3):235-237. |
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| 作者姓名： | 黄柏青 刘曼西 |
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| 作者单位： | 1. 华中科技大学生命科学与技术学院,湖北省卫生职工医学院,
2. 华中科技大学生命科学与技术学院, |
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| 摘 要： | 研究了大丽轮枝菌（Verticillium dahiliae Kleb.)激 发子V44和酵母提取物分别诱导丹参（Salvia miltiorrhiza)毛状根和丹参转化细胞后，过氧化物酶活力的变化，结果表明，诱导后过 氧化物酶活力显著提高，丹参毛状根酶活力变化大于丹参转化细胞，两种植物材料过氧化物酶活力变化与诱导浓度和诱导时间有关，实验提示，丹参毛状根可能对诱导物刺激具有更好的 抗性，且有利于次生代谢产物的积累。
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| 关 键 词： | 丹参 毛状根 转化细胞 过氧化物酶活力 真菌诱导物 药用植物 抗病性 组织培养 |
| 文章编号： | 1004-3888(2001)03-0235-03 |
| 修稿时间： | 2001年2月22日 |
Effects of Fungal Elicitors on Peroxidase Activities in Hairy Root Cultures and Transformed Cells of Salvia miltiorrhiza |
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| HUANG Bai qing ,LIU Man xi.Effects of Fungal Elicitors on Peroxidase Activities in Hairy Root Cultures and Transformed Cells of Salvia miltiorrhiza[J].Journal of Hubei Agricultural College,2001,21(3):235-237. |
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| Authors: | HUANG Bai qing LIU Man xi |
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| Institution: | HUANG Bai qing 1,2,LIU Man xi 1 |
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| Abstract: | Hairy root cultures of Salvia mlitiorrhiza and transformed Salvia miltiorrhiza cell suspension cultures were induced by Verticillium dahliae V44 and yeast extract respectively. The activities of peroxidase of the hairy root and the transformed cell increased significantly. Results showed that the elicitors from different sources, different concentration and different duration had different effects on the enzymes. The changes of peroxidase activities from hairy root cultures were higher than that from cell suspension cultures. It should be beneficial for its adversity resistance and the accumulation of secondary metabolites in hairy root cultures of Salvia miltiorrhiza. |
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| Keywords: | hairy root cultures of Salvia miltiorrhiza transformed Salvia miltiorrhiza cell suspension cultures peroxidase activity fungal elicitors |
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