Attachment of Malassezia pachydermatis to the ear dermal cells in canine otitis externa. |
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| Authors: | A Masuda T Sukegawa H Tani T Miyamoto K Sasai Y Morikawa E Baba |
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| Institution: | Veterinary Internal Medicine, Department of Veterinary Medicine, Graduate School of Agriculture and Biological Sciences, Osaka Prefecture University, Sakai, Japan. |
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| Abstract: | To investigate the predominance of Malassezia pachydermatis (M. pachydermatis) as a causative agent of canine otitis externa, ear cerumen samples were observed for adhesion of M. pachydermatis to the cornified epithelial cells by light and electron microscopes. The yeasts appeared not to adhere to the cornified epithelial cells directly, but they seemed to exist in the proximity of the epithelial cells with an electron opaque halo-like space around them. Polysaccharide and lipid staining techniques were conducted to identify the substances existing in that space. Lipid substances, not saccharides, were observed around the yeasts and the cornified epithelial cells. These results suggested that in the canine ear canal malassezia yeast attachment to the cornified epithelial cells is mediated by lipids. |
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