| 玻璃化法超低温保存匍匐翦股颖胚性愈伤组织及其植株再生 |
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| 引用本文： | 李红民,马晖玲.玻璃化法超低温保存匍匐翦股颖胚性愈伤组织及其植株再生[J].甘肃农业大学学报,2009,44(4). |
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| 作者姓名： | 李红民 马晖玲 |
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| 作者单位： | 甘肃农业大学草业学院,甘肃,兰州730070 |
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| 基金项目： | 教育部"春晖计划"项目 |
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| 摘 要： | 采用玻璃化法对匍匐翦股颖愈伤组织进行超低温保存条件及植株再生进行初步研究.结果表明:将继代2次的愈伤组织接种到预培养基上,4℃低温预培养5 d.预培养后的愈伤组织在常温下用装载液过渡10 min,再于0℃下用玻璃化溶液PVS2处理50 min后,加入新鲜的PVS2迅速投入液氮中保存1 h以上,取出后在30℃水浴中解冻,用MS+1.0 mol.L-1蔗糖溶液洗涤3次,每次10 min,细胞存活率可达85.6%,接种在恢复培养基上培养,胚性愈伤组织能正常进行体细胞胚胎发生、成熟和植株再生.
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| 关 键 词： | 匍匐翦股颖 愈伤组织 玻璃化法 超低温保存 |
Cryopreservation of creeping bentgrass embryogenic callus by vitrification and its plant regeneration |
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| LI Hong-min,MA Hui-ling.Cryopreservation of creeping bentgrass embryogenic callus by vitrification and its plant regeneration[J].Journal of Gansu Agricultural University,2009,44(4). |
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| Authors: | LI Hong-min MA Hui-ling |
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| Institution: | LI Hong-min,MA Hui-ling(College of Prataculture,Gansu Agricultural University,Lanzhou 730070,China) |
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| Abstract: | The procedure for cryopreservation by vitrification was preliminarily developed in creeping bentgrass callus.The results showed that the callus were inoculated into pre-culture medium after twice successive transfer,cultured under 4 ℃ for five days.Then the callus were put into loading solution for 10 min under room temperature,and were exposed to PVS2 for 50 min at 0 ℃,after changing the solution with fresh PVS2,the callus were put into the liquid nitrogen for at least 1 h.Taken out the callus and put them into 30 ℃ water,after defrosting,they were washed by the MS medium with 1.0 mol·L-1 sucrose for 3 times,each time 10 min.The survival rate of cells reached to 85.6 %.Then the callus were transferred into the recovering medium,somatic embryogenesis and plantlets regeneration from embryogenic callus could be induced. |
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| Keywords: | creeping bentgrass callus vitrification cryopreservation |
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