| 培养基配方对盾叶薯蓣组培苗生根的影响研究 |
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| 引用本文： | 张金莲,李少峰,王军,董新玉,孔宁忠,张西露,杨芬.培养基配方对盾叶薯蓣组培苗生根的影响研究[J].西南农业学报,2006,19(3):506-509. |
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| 作者姓名： | 张金莲 李少峰 王军 董新玉 孔宁忠 张西露 杨芬 |
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| 作者单位： | 大理州经济作物科学研究所,云南,宾川,671600 |
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| 摘 要： | 本文对影响盾叶薯蓣组培苗生根的主要因素:MS1、/2 MS基本培养基、活性碳、NAA、6-BA、AgNO3进行了试验研究。结果表明在诱导盾叶薯蓣组培苗生根过程中,基本培养基以MS为佳,活性碳对生根具明显抑制作用。NAA与6-BA具互作效应,诱导盾叶薯蓣组培苗生根时应尽量减少(或不用)6-BA,培养基以MS NAA1.0 6-BA0.25效果最佳。AgNO3对诱导盾叶薯蓣组培苗生根无明显作用,并与NAA不存在互作效应。
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| 关 键 词： | 盾叶薯蓣 组培苗 生根 MS、1/2MS基本培养基 活性碳 NAA 6-BA AgNO3 |
| 文章编号： | 1001-4829（2006）03-0506-04 |
| 收稿时间： | 2005-03-15 |
| 修稿时间： | 2005-03-15 |
Effects of culture media on root-growing of Dioscorea zingiberensis seedling |
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| ZHANG Jin-lian,LI Shao-feng,WANG Jun,DONG Xin-yu,KONG Ning-zhong,ZHANG Xi-lu,YANG Fen.Effects of culture media on root-growing of Dioscorea zingiberensis seedling[J].Southwest China Journal of Agricultural Sciences,2006,19(3):506-509. |
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| Authors: | ZHANG Jin-lian LI Shao-feng WANG Jun DONG Xin-yu KONG Ning-zhong ZHANG Xi-lu YANG Fen |
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| Institution: | Dali Economic Crops Research Institute, Yunnan Binehuan 671600, China |
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| Abstract: | The effects of MS,1/ 2MS culture medium and medium additive such as activated charcoal,NAA,6-BA and AgNO_3 on root-growing of Dioscorea zingiberensis seedling were studied.Results indicated that MS was a little better than 1/?2MS in root-growing,and no effect was observed for AgNO_3,but activated charcoal showed strong inhibition.In addition,NAA and 6-BA has significant interaction,therefore,the rate of 6-BA should be minimized in practical use.The best formula for root-growing induction was MS NAA(1.0 mg/L) 6-BA(0.25 mg/L). |
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| Keywords: | Dioscorea zingiberensis tissue culture seedling root-growing culture medium |
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