Varietal discrimination and genetic relationships of Vitis vinifera L. cultivars from two major Controlled Appellation (DOC) regions in Portugal |
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| Authors: | Isaura Castro Juan Pedro Martín Jesús Maria Ortiz Olinda Pinto-Carnide |
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| Institution: | 1. Centro de Genómica e Biotecnologia da Universidade de Trás-os-Montes e Alto Douro, Instituto de Biotecnologia e Bioengenharia (CGB-UTAD/IBB), 5001-801 Vila Real, Portugal;2. Departamento de Biología Vegetal, Escuela Técnica Superior de Ingenieros Agrónomos, Universidad Politécnica de Madrid, Ciudad Universitária s/n, 28040 Madrid, Spain |
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| Abstract: | Thirty-nine grapevine cultivars widely grown in Portugal, especially in Vinhos Verdes and Douro regions, and two well known international cultivars as standards, were genotyped at 12 microsatellite loci. The number of alleles per locus ranged from 6 to 12, and the number of allelic combinations per locus from 13 to 26. The total number of unique genotypes in the 12 analysed loci was 120, having most of the cultivars (38 out of 41) at least one unique genotype in any of the loci. The microsatellite profiles were adequate to discriminate 41 cultivars. The level of observed heterozygosity at each locus varied from 70.7% to 95.1%. VVMD28 has been revealed as one of the most informative markers. Several synonymies between Spanish and Portuguese cultivars were confirmed, and some homonymies are discussed. The genetic profiles of all 41 cultivars were searched for possible parent-offspring groups. The data obtained revealed the possible descendence of Touriga Franca from Touriga Nacional and Marufo. |
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| Keywords: | Biodiversity Grapevine germplasm Pedigree SSRs Varietal identification |
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