Development of ITS sequence based SCAR markers for discrimination of Paphiopedilum armeniacum, Paphiopedilum micranthum, Paphiopedilum delenatii and their hybrids |
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| Authors: | Yung Wei Sun Yu Ju Liao Yin Sui Hung Jia Ci Chang Jih Min Sung |
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| Institution: | 1. Taiwan Seed Improvement and Propagation Station, Taichung County 42642, Taiwan, ROC;2. Department of Food Science and Technology, Hung Kuang University, Taichung County 43302, Taiwan, ROC |
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| Abstract: | Paphiopedilum armeniacum, Paphiopedilum micranthum and Paphiopedilum delenatii are endangered orchid species. These three Paphiopedilum species and their hybrids are difficult to distinguish morphologically. In this study, rDNA-ITS (internal transcribed spacer) sequences were used to design species-specific SCAR (sequence characterized amplified regions) markers to distinguish P. armeniacum, P. micranthum, P. delenatii and their respective hybrids. The developed markers efficiently amplified 600 bp DNA product for P. armeniacum and its hybrids (SCAR-600armF/Pap-ITS2R), 300 bp product for P. delenatii and its hybrids (SCAR-300delF/Pap-ITS2R) and 700 bp product for P. micranthum and its hybrids (SCAR-700micF/Pap-ITS2R). The effectiveness of designed species-specific markers was also confirmed by using multiplex polymerase chain reaction amplification with a combination of developed three SCAR markers. |
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| Keywords: | ITS Paphiopedilum armeniacum Paphiopedilum micranthum Paphiopedilum delenatii SCAR marker |
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