Effect of cytokinin content of the regeneration media on in vitro rooting ability of adventitious apple shoots |
| |
| Authors: | K Magyar-Tábori J DobránszkiI Hudák |
| |
| Institution: | Research Institute of Nyíregyháza, Research Institutes and Study Farm, Center for Agricultural and Applied Economic Sciences University of Debrecen, P.O. Box 12, H-4400, Hungary |
| |
| Abstract: | Rooting ability of ‘Royal Gala’ shoots regenerated on media with different cytokinin content (thidiazurone, benzyladenine, benzyladenine-riboside and meta-topolin-riboside) was observed directly after regeneration and compared after subculture on hormone-free medium (A), or on medium with decreased cytokinin content (B), or elevated gibberellic acid (GA3) content (C) for a week, or after a subculture on proliferation media (1.0 mg l−1 benzyladenine-riboside) for four weeks (treatment D). Rooting and acclimatization of regenerants after directly regeneration was not successful. Subculture of shoots on hormone-free medium did not improve the rooting ability of shoots, while their subculture on B and C media resulted in up to 36% rooting rate depending on regeneration media from which the shoots originated. The best rooting rate (up to 76%) was achieved in shoots regenerated on medium with benzyladenine-riboside after treatment D similarly to rooting ability of micropropagated shoots (80%). The rooting capacity of shoots depended on both the cytokinin content of the regeneration media and different subculture media used. All rooted shoots survived after acclimatization. |
| |
| Keywords: | BA benzyladenine BAR benzyladenine-riboside GA3 gibberellic acid IBA indol-3-butyric acid TDZ thidiazurone TOP meta-topolin TOPR meta-topolin-riboside |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
