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Pericarp total protein profiles as molecular markers of tomato fruit quality traits in two segregating populations
Authors:Gustavo R Rodríguez  Javier H Pereira da Costa  Damian D Tomat  Guillermo R Pratta  Roxana Zorzoli  Liliana A Picardi
Institution:1. Cátedra de Genética, Facultad Ciencias Agrarias, Universidad Nacional de Rosario, CC 14 (S2125ZAA) Zavalla, Santa Fe, Argentina;2. Consejo Nacional de Investigaciones Científicas y Técnicas, Argentina;3. Consejo de Investigaciones de la Universidad Nacional de Rosario, Argentina
Abstract:The tomato fruit quality results of biochemical and physiological changes that occur during the ripening process. Although, the pericarp total protein profiles are less polymorphic than DNA-based markers the polymorphism in those could be directly associated with fruit quality traits. The aim of this work was to identify associations between polymorphic polypeptides from fruit pericarp at two ripening stages and fruit quality traits evaluated in two segregating populations of tomato. A cross between a normal ripening cultivar of Solanum lycopersicum (C, Caimanta) and a genotype carrying the nor (non ripening) gene (N) as well as a cross between Caimanta and a cherry type tomato of S. lycopersicum var. cerasiforme (Ce) showed genetic variance for several fruit quality traits such as fruit weight, shape, solids soluble content, acidity, color and fruit shelf life. The quantitative variations observed at phenotypic level had correspondence with the polymorphism detected in the protein profiles. Indeed, the polymophic polypeptides associated with quality fruit traits and fruit shelf life would be useful to assist tomato breeding programs as protein molecular markers.
Keywords:Plant breeding  SDS-PAGE  S  lycopesicum var  cerasiforme  Shelf life
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