Exploitation of SSR,SRAP and CAPS-SNP markers for genetic diversity of Citrus germplasm collection |
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| Authors: | Mohamed Hamdy Amar Manosh Kumar Biswas Zongwen Zhang Wen-Wu Guo |
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| Institution: | 1. National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, Hubei Province, China;2. Egyptian Deserts Gene Bank, Desert Research Center, North Sinai, Egypt;3. Bioversity International, Rome, Italy |
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| Abstract: | The present study was to assess informativeness and efficiency of three different molecular markers for genetic diversity among 24 Citrus and its relative species. Sixty one SSR, 33 SRAP and 24 CAPS-SNP markers were used to evaluate the level of polymorphism and discriminating capacity. A total of 596, 656 and 135 polymorphic amplicons were observed in SSR, SRAP and CAPS-SNP markers with average polymorphism information content (PIC) of 0.97, 0.98 and 0.89, respectively. High levels of polymorphism were recorded for SSR and SRAP compared with CAPS-SNP markers. The highest correlations (r = 0.930) were obtained between SSR and SRAP markers, whereas SSR and CAPS-SNP were poorly correlated (r = 0.833). Cluster analysis was performed to construct dendrograms using UPGMA. And the dendrogram from SSR data was most congruent with the general dendrogram. These findings provide basis for future efficient use of these molecular markers in the genetic analysis of Citrus and its relatives. |
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| Keywords: | Citrus SSR SRAP CAPS-SNP PIC Genetic diversity |
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